摘要
[目的]探讨建立肠易激综合征(IBS)动物模型的方法,并评价其内脏敏感性变化。[方法]采用慢性束缚、夹尾刺激作为应激原诱导IBS大鼠模型,检测其肠道运动(结肠排便时间、排便颗粒)和感觉(腹壁收缩反射、腹壁肌电活动),并以逍遥散及匹维溴胺进行药物反证。[结果]与正常对照(对照)组比较,慢性束缚、夹尾刺激诱导IBS大鼠结肠转运效应明显增高(P<0.01),在1.0、1.5、2.0 ml不同扩张容量下腹壁收缩反射评分及腹壁肌电活动明显增高(P<0.05,<0.01)。而经药物治疗后,可明显降低大鼠结肠转运功能及内脏高敏感性(P<0.05,<0.01)。[结论]慢性束缚、夹尾刺激可以引起大鼠肠道运动增强,内脏敏感性增高,而肠黏膜未见异常病理改变,符合IBS的基本特征,可用于实验研究。
[Objective]To study the methods for establishing the animal model of IBS rats and evaluate the visceral sensitivity, [Methods]The stressed IBS model rats were made by chronic bandaged-stress and inserting the end. The colon motion ,the AWR of colorectal disention and the abdominal electrical activity were observed. The regulation of Xiaoyaosan and pinaverium bromide were observed. [Results] Chronic bandaged-stress and inserting the end increased the colon motion and visceral sensitivity（P〈0. 05,〈0. 01）, while Xiaoyaosan and pinaverium bromide could decrease the colon motion and visceral hypersensitivity of IBS rats （P〈0. 05, 〈0. 01）. [Conclusion ]The animal model established by chronic bandaged-stress and inserting the end accords with the clinical characteristics of IBS, can be used in experimental Study.
出处
《中国中西医结合消化杂志》
CAS
2008年第2期87-90,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
浙江省中医药科技计划(2006C025)
关键词
肠易激综合征
模型
内脏高敏感性
irritable bowel syndrome（IBS）
model
visceral hypersensitivity
