强骨饮对去卵巢大鼠腰椎骨形态计量学的影响

Effect of Qianggu drink on bone histomorphometry of lumbar vertebrae in ovariectomized rats

目的:观察自拟强骨饮对骨质疏松症的骨形态计量学的影响,并探讨其机制。方法:选健康雌性SD大鼠60只,体重230～280g。随机分为3组,强骨饮组(治疗组)、密钙息组(对照组)和空白组,每组20只。分别采取切除双侧卵巢方法进行骨质疏松造模,10周造模成功后,开始给药,治疗组用自拟强骨饮灌胃,每日1次,每次0.001ml/kg;对照组用密钙息皮下注射,每日1次,每次0.72U/kg。空白组,不做处理。在给药后45、90、135及180d每组各取5只大鼠进行检测,先测量体重,然后空气栓塞处死,分离腰椎,获得腰椎样本,经切片等处理后,显微镜下作骨形态计量学检测(包括骨体积分数、骨小梁厚度、骨小梁间距)。结果:给药后135d治疗组骨小梁厚度与对照组相比差异有统计学意义。给药后180d治疗组骨小梁厚度、骨小梁间距、骨体积分数与对照组相比均具有统计学意义。治疗组与空白组相比骨小梁厚度、骨小梁间距、骨体积分数差异均具有统计学意义。结论:自拟强骨饮可以明显改善骨形态计量学指标,可能是通过刺激成骨细胞生长,抑制破骨细胞活性,并抑制高骨转换趋势来实现的。

Objective：To observe the effect of Chinese herbal medicine Qianggu drink（强骨饮） on bone histomorphometry of lumbar vertebrae in ovariectomized rats and to explore its mechanism. Methods：Sixty female SD rats（weight 230 to 280 gram） were randomized into three groups：Qianggu drink（强骨饮） group（treatment group）,Miacalcic group（control group）and blank group. The osteoporosis model was established successfully after all rats were ovariectomized for 10 weeks. The treatment group was used intragastric infusion with Qianggu drink （强骨饮） 0.001 ml/kg once a day. The control group was used hypodermic injection once a day with Miacalcic 0.72 U/kg. Five rats were killed respectively and the lumbar specimens were taken and cut for bone histomorphometric analysis at 45,90,135 and 180 days after the treatment. Results：The thickness of trabecular bone in treatment group had the statistical significance with control group at 135 days after ovariectomized rats were given the medicine. The data of BV/TV and the thickness and space of trabecular bone had statistical significance compared with control group and blank group 180 days later. Conclusion：Qianggu drink （强骨饮） could effectively improve the histomo-rphometry indexes. The mechanism probably plays by stimulating osteogenesis and inhibiting the cytoactive of osteoclast and the trend of bone transform.