sICAM-1在重症肌无力患者血清及外周血单个核细胞培养上清液中的变化

The Serum and Cultured-cell Supernatant Level of slCAM-1 in Myasthenia Gravis

目的:了解可溶性细胞间黏附分子-1(sICAM-1)在重症肌无力(MG)患者血清及外周血单个核细胞(PBMC)培养上清液中的变化情况。方法:用酶联免疫吸附法(ELISA)检测正常对照和MG患者,血清及PBMC培养上清液中sICAM-1的水平,并分析激素对上清液中sICAM-1的影响。结果:正常对照组与MG组血清sICAM-1的水平没有显著差异;而在PBMC培养上清液中,MG组高于正常对照组。加入地塞米松培养48h后,7例MG患者PBMC培养上清液中sICAM-1水平降低。结论:MG患者PBMC可分泌更多的sICAM-1,升高的sICAM-1可能是体内ICAM-1产生及循环过程的中间产物;ELISA检测MG患者PBMC培养上清液(加FBS)中sICAM-1的水平是一种有效的检测手段;此外,地塞米松可以下调PBMC分泌sICAM-1。

Aim： To study the level of ICAM-1, especially soluble ICAM-1, in the serum and the supernatant of PBMC in patients with myasthenia gravis（MG）. Methods： We use ELISA to quanlificate the level of sICAM-1 in the serum and in PBMC culture supernatant of patients with MG and the healthy controls. Results： There was no significant difference in the serum level of sICAM-1 between the patients with MG and the healthy controls. While the level of sICAM-1 in the supernatant of the patients with MG was higher than that in healthy control. The supernatant level of sICAM-1 of seven patients with MG co-cultured with dexamethasone was decreased after 48 hours. Conclusion： PBMC secretes more sICAM-1 in MG patients than in normals. It is a better method to analyze the supernatant level of sICAM-1 in PBMC supernatant cultured with FBS in patients with MG. Dexamethasone can down-regulate the secretion of sICAM-1.