耐碳青酶烯类铜绿假单胞菌相关耐药基因及Ⅰ型整合酶基因研究

Drug Resistance Related Gene of Carbopenem Resistant Pseudomonas aeruginosa and Type Ⅰ Integrating Enzyme Gene

目的研究耐碳青酶烯类铜绿假单胞菌的相关耐药基因及Ⅰ型整合酶基因。方法采用聚合酶链反应(PCR)法对铜绿假单胞菌碳青酶烯类耐药相关的金属β-内酰胺酶IMP、VIM、SPM、GIM基因和外膜蛋白oprD2基因及Ⅰ型整合酶基因等6种主要耐药基因进行检测与分析。结果51株耐亚胺培南和美罗培南铜绿假单胞菌SPM、GIM金属酶基因检测均阴性,16株IMP和5株VIM型金属酶基因阳性,14株耐亚胺培南铜绿假单胞菌oprD2基因阳性,其余37株铜绿假单胞菌oprD2基因阴性,7株产金属酶并同时伴有膜孔蛋白oprD2基因缺失,49株Ⅰ型整合酶基因intⅠ1阳性。结论膜孔蛋白oprD2基因缺失是铜绿假单胞菌对碳青酶烯类抗菌药物耐药的主要原因,其次是产生金属酶,Ⅰ型整合酶基因广泛存在于铜绿假单胞菌中,提示要加强对耐药基因在病原菌种属间传播和扩散的监测工作。

OBJECTIVE To sudy drug zesistance related gene of carbopenem resistant Pseudomonas aeruginosa and type 1 integrating enzyme gene. METHODS With PCR method to detect and analyze carbopenem resistant P. aeruginosa pertinent metal β-lactamase IMP, VIM, SPM, GIM genes and cell membrane protein oprD2 gene and six main drug resistance genes of type I integrating enzyme gene and five others. RESULTS Fifty one strains of imipenem and meropenem resistant P. aeruginosa SPM, GIM metal enzyme genes were detected to be negative, 16 strains of IMP and 5 strains of VIM type metal enzyme were all positive, 14 strains of carbopenem resistant P. aeruginosa oprD2 gene were positive, other 37 strains of P. aeruginosa oprD2 gene were negative, 7 strains produced metal enzyme and cell membrane protein oprD2 gene were deleted at the same time,49 strains with type I integrating enzyme gene int I 1 were positive. CONCLUSIONS It is indicated the gene deletion of P. aeruginosa outer membrane protein OprD2 is important mechanism of carbopenem resistant P. aeruginosa, the second is metal enzyme, type I integrating enzyme exists largely in P. aeruginosa. This hints the monitoring that drug resistance gene spreads and propagates in bacterium strain must be reinforced.