黑绛丹对放射所致大鼠皮肤溃疡的作用

Effect of Heijiang Pill on Radiation Skin Ulcer in Rats

目的探讨60Co源一次性照射剂量与大鼠放射性溃疡程度的关系,观察黑绛丹对大鼠放射性皮肤溃疡的治疗作用。方法雌性Wistar大鼠66只随机分为60Gy、40Gy照射组各30只,空白对照组6只。60Co源一次性照射右后腿,记录溃疡出现时间、程度。60 Gy、40Gy照射组再分别分为模型组、黑绛丹治疗组和利凡诺治疗组3个亚组,每组各10只;溃疡形成后各组大鼠分别给予相应的处理,观察溃疡发生率、病理改变、愈合率及愈合时间,观察期为90天。结果与40Gy照射组比较,60Gy照射组大鼠放射性皮肤溃疡的发生率明显增高,且溃疡出现时间早(P<0.05)。同一剂量组中,黑绛丹组皮肤溃疡治愈率均明显高于利凡诺组(P<0.05);在40Gy组中,与利凡诺组比较,黑绛丹组溃疡平均愈合时间明显缩短,差异有统计学意义(P<0.05),且40Gy黑绛丹组溃疡治愈率明显高于60Gy黑绛丹组(P<0.05)。病理观察证实黑绛丹对放射损伤局部上皮细胞有增殖、修复的作用。结论随放射剂量增加,放射性皮肤溃疡的出现时间提早,且溃疡程度加重。黑绛丹可有效治疗放射性皮肤溃疡,对低放射剂量大鼠溃疡模型疗效更加显著。

Objective To investigate the relationship between single dosage of ^60Co radiation and the degree of radiation-induced skin ulcers, and to evaluate the curative effect of Heijiang Pill （HJP） on skin ulcer induced by various dosages of radiation in rats. Methods Sixty-six Wistar female rats were randomly divided into three groups, the blank control group （n =6） and the two radiation groups, each 30 rats, with their right hind leg exposed respectively to 60 Gy and 40 Gy of ^60 Co radiation. The time of emergence and degree of skin ulcer were recorded. Then rats in the two radiation groups were subdivided into the HJP group, the Ethacridine group and the model group, 10 in each group, they received corresponding treatment after ulceration, and the incidence, pathology, cure rate and cure time of skin ulcer were observed in the 90 days of observation. Results The incidence of skin ulcer was higher and occurred earlier in rats radiated with 60 Gy than that with 40 Gy （P 〈 0.05）. In the same radiation dosage, the ulcer healing rate in rats treated with HJP was higher than that treated with Ethacridine （P 〈0.05） , and in the subgroups of 40 Gy radiation, the mean cure time in the HJP group was shorter （P 〈 0. 05 ）. The local epithelial proliferation and repairing could be verified by pathological observation in the HJP group. Conclusion Larger dosage of radiation leads to earlier emergence and aggravation of ulcers. HJP can effectively cure radiation skin ulcer, and the effect is especially significant on the ulcer induced by low dose radiation.