麝香石竹的组培脱毒技术研究

Study on the Detoxification Technology by Tissue Culture of Carnation

[目的]探索麝香石竹的组培脱毒技术。[方法]以麝香石竹0.3-0.4 mm长的茎尖为外植体,在分化培养基中诱导丛生芽,丛生芽进行继代培养后,将幼苗进行病毒检测,将无毒幼苗保留继续繁殖,然后将无毒的幼苗移植到生根培养基中诱导生根。[结果]不同大小的香石竹茎尖对丛生芽的诱导有较大影响,以0.3 mm茎尖为最佳。丛生芽的诱导以MS＋6-BA 2.0 mg/L＋IAA 2.0 mg/L、根的诱导以1/2MS＋IAA 0.5 mg/L（或IBA 0.5 mg/L）为最佳培养基。田间比较试验表明,与未脱毒麝香石竹相比,脱毒麝香石竹花的颜色鲜艳,切花品质好,产花量高,花裂萼少。[结论]该研究为扩大麝香石竹的繁殖提供了技术保障。

[Objective] The aim was to explore the detoxification technology by tissue culture of carnation.[Method]Taking shoot tips with length of 0.3～0.4 mm of carnation as explants,the clustered buds were induced on the differentiation medium,the virus detection was conducted on the seedlings after the subculture was conducted on the clustered buds.The virus-free seedlings were remained and propagated continuously and then they were transplanted onto rooting medium for rooting induction.[Result] The carnation shoot tips with different sizes had igger influences on the induction of clustered buds and the shoot tips with length of 0.3 mm were optimum.MS＋6-BA 2.0 mg/L＋IAA 2.0 mg/L was the optimum medium for the induction of clustered buds and 1/2MS＋IAA 0.5 mg/L（or IBA 0.5 mg/L） was the optimum medium for the induction of root.The field comparative trial showed that compared with the non detoxification carnation,the flower color of detoxified carnation was more brilliant,its quality of cut flower was better,its flowe r yield was higher and its cleft calyx was less.[Conclusion] The study provide technical guarantee for expanding the propagation of carnation.