多壁碳纳米管致A549细胞毒性与氧化损伤的研究

In vitro cytotoxicity and oxidative damage effects of Multi-wall carbon nanotube on A549 cell

目的探讨多壁碳纳米管(MWCNTs)对人Ⅱ型肺泡上皮细胞(AT-Ⅱ)A549细胞的体外细胞毒性和氧化损伤作用。方法用脱氧核糖核酸钠盐(deoxyribonucleic acid sodium salt,DNA钠盐)提高MWCNTs的分散度,设4个浓度组(2.5、10、25和100μg/ml)、DNA钠盐溶剂对照组和生理盐水对照组,染毒24h后,用MTT法观察细胞毒性,并根据染毒后总蛋白(TP)、乳酸脱氢酶(LDH)、一氧化氮(NO)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和丙二醛(MDA)的变化情况分析MWCNTs的细胞毒性和氧化损伤作用。结果25、100μg/ml浓度组细胞存活率及细胞毒性和氧化损伤指标均有不同程度的改变,呈一定的暴露-反应关系;随着染毒剂量的升高,细胞和其上清液中TP、LDH、NO和MDA含量随之升高,而GSH和SOD含量随之降低,且呈一定的暴露-反应关系;且与细胞发生融解破碎,间隙加大等形态学改变情况相符。结论MWCNTs对A549细胞产生一定的毒性和氧化损伤作用,且随剂量的增大而增强。

Objective To study the in vitro cytotoxicity and oxidative damage effects of Multi-wall Carbon Nanotube on A549 cells. Methods The A549 cells were treated with different concentrations of MWCNTs （2.5,10,25,100μg/ml）, slovent control and saline control for 24 h. MTr assays were applied for testing A549 cell activity in vitro, total protein （TP）, lactate dehydrogenase（ LDH）, nitrogen monoxidum （NO）, glutathione （ GSH ）, superoxide dismutase （ SOD）, and malondialdehyde （MDA） in supernate and intra-cellular were determined to evaluate the in vitro cytotoxicity and oxidative damage effects of NWCNTs on A459 cells. The morphology changes of A549 cells were also observed. Results In comparison with control, the viabilities of A549 cells were decreased significantly after exposure to MWCNTs. The level of TP,LDH ,NO and MDA in exposed groups were higher, while GSH and SOD level were lower than control with a significant dose-effect relationship, which accord to the morphology changs of A549 cells exposed to MWCNTs. Conclusion MWCNTs induces cytotoxity and oxidative stress on A549 cells. With the increases of the concentration of MWCNTs.