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POLH基因实时荧光定量PCR检测条件的建立 被引量：1

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作者 胡恭华 庄志雄 董明华 庾蕾 黄海燕 纪卫东
机构地区 赣南医学院预防医学系 深圳市疾病预防控制中心 中山大学公共卫生学院预防医学系
出处 《毒理学杂志》 CAS CSCD 北大核心 2008年第2期143-145,共3页 Journal of Toxicology
基金 国家重点基础研究发展计划(973)项目(2002CB512903 2002CB512904)
关键词 SYBR Green I 实时荧光定量PCR POLH
分类号 R994.6 [医药卫生—毒理学]
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参考文献8

  • 1Bengtsson M, Karlsson H J, Westman G, et al. A new minor groove binding asymmetric cyanine reporter dye for real-time PCR. Nucleic Acids Res,2003,31 : 45.
  • 2Masutani C, Kusumoto R, Yamada A, et al. The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase η- Nature, 1999, 399:700-704.
  • 3Johnson RE, Kondratick CM, Prakash S. hRAD30 mutations in the variant form ofxeroderma pigmentosum. Science, 1999,285: 263-265.
  • 4Walker NJ. Real-time and quantitative PCR: applications to mechanism-based toxicology .J Biochem Mol Toxicol, 2001,15: 121-127.
  • 5Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-△△CT method. Methods, 2001,25: 402-408.
  • 6Yin JL, Shackel NA, Zekry A, et al. Real-time reverse- transcriptase polymerase chain reaction ( RT-PCR ) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green Ⅰ. Immunol Cell Biol, 2001,79 : 213-221.
  • 7Brownie J, Shawcross S, Theaker J, et al. The elimination of primer-dimer accumulation in PCR. Nucleic Acids Res, 1997, 25 : 3235-3241.
  • 8Halford WP, Falco VC, Gebhardt BM, et al. The inherent quantitative capacity of the reverse transcription-polymerase chain reaction. Anal Biochem, 1999,266:181-191.

同被引文献37

  • 1杨淞,卢迈新,黄樟翰,高风英.5种杂交F_1罗非鱼生长性能比较研究[J].淡水渔业,2006,36(4):41-44. 被引量：29
  • 2HOWARD A D,FEIGHNER S D, CULLY D F,et al. A receptor in pituitary and hypothalamus that functions in growth hormone release [ J ]. Science, 1996A ,273:974 - 977.
  • 3KOJIMA M, HOSODA H,DATE Y,et al. Ghrelin is a growth-hormone-releasing acylated peptide from stomach [ J ]. Nature, 1999,402:656 - 660.
  • 4TAKAYA K, ARIYASU H, KANAMOTO N, et al. Ghrelin strongly stimulates growth hormone release in humans[J]. Clin Endocrinol Metab,2000, 85:4908 -4911.
  • 5UNNIAPPAN S, LIN X, CERVINI L, et al. Goldfish ghrelin: molecular characterization of the complementary deoxyribonucleic acid, partial gene structure and evidence for its stimulatory role in food intake [ J ]. Endocrinol,2002,143:4143 - 4146.
  • 6KAIYA H, KOJIMA M, HOSODA H, et al. Amidated fish ghrelin purification,cDNA cloning in the Japanese eel and its biological activity [ J ]. Endocrinol,2003, 176:415 -423.
  • 7KAIYA H, KOJIMA M, HOSODA H, et al. Identification of tilapia ghrelin and its effects on growth hormone and prolactin release in the tilapia,Oreochromis mossambicus [ J ]. Comp Biechem Physiol,2003 ,135 :421 - 429.
  • 8PARHAR IS,SATO H,SAKUMA Y. Ghrelin gene in cichlid fish is modulated by sex and development [ J ]. Biochem Biophys Research communications ,2003,305 : 169 - 175.
  • 9KAIYA H, KOJIMA M, HOSODA H, et al. Peptide purification, complementary deoxyribonucleic acid (DNA) and genomic DNA cloning, and functional characterization of ghrelin in Rainbow Trout [ J]. Endocrinol, 2003,144(12) :5215 -5226.
  • 10YEUNG C M, CHAN C B, WOO N Y, et al. Seabream gbrelin:cDNA cloning,genomic organization and promoter studies [ J ]. Endocrinol,2006,189 : 365 - 379.

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毒理学杂志
2008年 第2期

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