摘要
目的探讨同种异体大鼠骨髓间充质干细胞移植在阿霉素诱导的扩张型心肌病心功能衰竭大鼠心脏内存活、分化的情况及对左心室功能的影响。方法雌性Wistar大鼠55只,随机分成正常对照组(n=10)、模型组(n=15)、诱导前移植组(n=15)和诱导后移植组(n=15)。体外分离培养雄性大鼠骨髓间充质干细胞,传至一代后用10μmol/L5-氮胞苷诱导4周,DAPI标记后,诱导前移植组移植诱导前的骨髓间充质干细胞,诱导后移植组移植诱导后的骨髓间充质干细胞;4周后检测左心室功能及移植细胞存活、分化情况。结果异体骨髓间充质干细胞移植4周后可存活并分化成心肌细胞,表达心肌细胞特异性蛋白;与模型组比较,细胞移植组左心室功能明显改善,且两细胞移植组间差异无显著性。结论同种异体骨髓间充质干细胞移植4周后可存活并分化成心肌细胞,对扩张型心肌病心功能衰竭大鼠的左心室功能有保护作用。
Aim To investigate the possibility of survival, differentiation of bone marrow mesenchymal stem cells （ BMMSC） of rats in host myocardium and the effect on left ventricular function in doxorubicin-induced heart failure. Methods 55 female Wistar rats were separated randomly into four groups： normal control group （n = 10）, heart failure group （ n = 15）, heart failure plus BMMSC transplantation group （n = 15 ）, heart failure plus induced BMMSC transplantation （ n = 15 ）. Male Wistar rats BMMSC were isolated and induced by 10μmol/L 5-aza for 4 weeks after first generation; then the cells were transplanted into rat heart by doxorubincinnduced heart failure after DAPI-labelled. After 4 weeks, left ventricular function were measured and the heart were checked by immunofluruscopy. Results BMMSC can survive and differentiate in host myocardium; the left ventricular function were significantly improved in BMMSC transplantation group compared with heart failure group, there was no difference between the two BMMSC transplantation groups. Conclusion BMMSC can survive in host myocardium after 4 weeks transplantation and can improve the left ventrieular function of doxorubinein-indueed dilated eardiomyopathy heart failure.
出处
《中国动脉硬化杂志》
CAS
CSCD
2008年第2期117-120,共4页
Chinese Journal of Arteriosclerosis
