摘要
以鸭肠炎病毒（DEV）SD-01株DNA为模板，根据GenBank上已发表的基因序列设计一对引物，利用PCR技术扩增出UE2全基因，将目的条带连入pGM—T载体，得到的阳性重组质粒命名为pGM—US2并进行序列测定。将测序结果与疫苗株、鸭瘟鸡胚化弱毒疫苗（C—KCE）株和单纯疱疹病毒-1型（HSV-1）、马立克氏病病毒（MDV）、伪狂犬病病毒（PRV）的US2基因同源性进行比较，发现核昔酸和氨基酸的序列同源性分别为100％～25．8％和100％～28％。结果表明，US2基因在DEV中是完全保守的，但与其它疱疹病毒相比同源性较低。
The genomic DNA of DEV SD -01 strain was extracted and a pair of primers was designed according to the published nucleotide sequence in GenBank. The US2 gene was amplified by polymerase chain reaction （PCR） with extracted total DNA from DEV SD -01 strain as template and cloned into vector pGMT. The positive recombinant plasmid was named pGM - US2 and sequenced. The results showed that the homology of the nucleotides and amino acid of US2 gene from DEV SD -01 strain with that of DEV vaccine strain , C - CKE strain, HSV - 1, MDV and PRV were 100% - 25.8% and 100% - 28% respectively. These data suggested that US2 gene was completely conserved among different DEV strains, whereas showing low level homology with other herpesviruses.
出处
《山东农业科学》
2008年第3期33-36,共4页
Shandong Agricultural Sciences
基金
山东省自然科学基金项目(编号:Y2007D70)
