肝癌组织相关基因表达与肝癌术后生存的关系

Relationship between HCC-related genes expression in tumor tissue and postoperative survival of patients with hepatocellular carcinoma

目的探讨肝癌组织内多个肝癌相关基因表达情况及其与术后生存预后的关系。方法对72例手术切除肝癌患者的追踪随访资料进行生存分析,分析各临床和病理指标与生存预后的关系。对手术切除的肝癌标本以半定量RT-PCR方法测定癌组织中多个基因的表达情况。结果术前肝功能分级、血清AFP、AFU、癌组织分化程度、有无癌栓与预后相关(P<0.05)。肝癌组织内COL1A2、Gem in in、IGFBP3、SPINK2表达与患者的癌组织分化程度相关。结论肝癌组织内COL1A2、Gem in in、IGFBP3和SPINK2基因表达为肝癌术后生存的主要相关分子生物学因素。

Objective To investigate the relationship between HCC-related gene expression and postoperative survival of patients with hepatocellular carcinoma. Methods Survival analysis was used to analyze the follow-up data of 72 cases with hepatocellular carcinoma （HCC） postoperation. Semiquantitative reverse transcription polymerase chain reaction （ RT-PCR） was performed to test the expression of 10 genes in tumor tissue. Results Child.grades of liver function, serum AFP levels, AFU, tumor thrombosis and the degrees of histological differentiation were involved with prognosis. Tumor thrombosis and the degrees of histological differentiation were the independent factors affecting the survival after operation. The expression of COL1A2, Geminin, IGFBP3 and SPINK2 was related to the histological differentiation. Conclusion The expression levels of COL1A2, Geminin, IGFBP3 and SPINK2 in liver cancer are the main factors affecting the postoperative survival time.