开放体系下稻草-白腐菌对染料的吸附脱色与降解

Removal of malachite green and crystal violet by adsorption on rice straw and biodegradation by white rot fungus

在开放体系下,研究了稻草-白腐菌对孔雀绿和结晶紫的吸附脱色与降解。结果表明,稻草能有效地对孔雀绿和结晶紫进行吸附脱色,其中稻草用量、粒径和pH对稻草吸附染料有一定的影响,在合适条件下稻草对孔雀绿和结晶紫的最大吸附量分别可达到31.89、52.98mg/g;在吸附孔雀绿和结晶紫的稻草中接入白腐菌,发现开放体系下白腐菌能对两种染料进行降解,6d后,孔雀绿和结晶紫的脱色率分别达到93.67%和80.00%;再生后的稻草基质对孔雀绿和结晶紫也有较好的吸附,最大再生率分别达到74.45%和43.34%。降解过程中白腐菌分泌酶的酶活力与染料及稻草基质降解没有直接的联系。

Malachite green and crystal violet were removed by adsorption （due to biosorption） on rice straw and then biodegraded by white rot fungus. The effects of liquid-solid contact （rotation rate）, biomass dose, dye concentration, size of the rice straw particle and pH on the adsorptive capacities were determined. The rice straw exhibited high capacities for the dyes, up to 31.89 mg/g of malachite green and 52.98 mg/g of crystal violet were observed in the study. The adsorbed dyes were degraded by the inoculated white rot fungus; 93.67% of the adsorbed malachite green and 80.00% of the adsorbed crystal violet was degraded in 6 days after incubation in an open system. The regenerated rice straw was effective for removing the two dyes again; the regeneration recovered 74.45% of the capacity for malachite green and 43.34% of the capacity for crystal violet. Removal of the dyes and the loss of biomass caused by the actions of white rot fungus were not well correlated with its enzyme activity.