依达拉奉对血管性痴呆大鼠海马组织SOD、MDA的影响

THE EFFECT OF EDARAVONE ON THE SOD AND MDA IN HIPPOCAMPAL TISSUE OF RAT WITH VASCULAR DEMENTIA

[目的]探讨依达拉奉对血管性痴呆大鼠海马组织SOD、MDA的影响。[方法]采用双侧颈总动脉永久结扎方法制备慢性前脑缺血大鼠模型,分依达拉奉治疗组,都可喜组和正常对照组,自手术7d起,依达拉奉治疗组皮下注射依达拉奉,每日1次给药,药量3mg/kg体重,共20d。都可喜组用都可喜灌胃,每日1次,20mg/kg体重,共20d,各组分别取1个月及2个月为时间点,处死取脑,测定大鼠海马组织SOD、MDA。[结果]提取各组大鼠海马组织线粒体后,SOD活性检测结果显示:术后1月、2月依达拉奉治疗组SOD活性比相应时间点模型组SOD活性高,差异有统计学意义(P﹤0.05)。与都可喜的作用相比差异无统计学意义(P﹥0.05),而且2个月的治疗效果好于1个月。术后1月、2月依达拉奉治疗组MDA分别低于相应时间点模型组,差异有统计学意义(P﹤0.05)。与都可喜的作用相当,而且2个月的治疗效果好于1个月。[讨论]依达拉奉可以使血管性痴呆大鼠海马组织MDA含量下降,SOD含量升高,提示依达拉奉具有明显的清除缺氧缺血后脑组织内自由基的作用。

[Objective] To explore the effect of edaravone on the SOD and MDA in hippocampal tissue of rat with vascular dementia. [Methods] By using the mode of rat with chronic proeerebrum isehemia model through permanent bilateral occlusion of both common carotid arteries, the Wistar rats were randomly divided into 3 groups as the control group, the Edsravone group （VD＋ Edaravone） and the Duxil group （VD＋ Duxil）. After operation for seven days, rats in the Edaravone group were hypodermic injected with Edaravone 3mg/kg one time per day for 20 days. Rats in the Duxil group received gastric perfusion with 20 mg/kg Duxil per day for 20 days. These rots were sacrificed to obtain the brain at the time of one month and two month, then the SOD antivities and MDA content in the rat hippoeampus were detected. [Results] The cbondriosome in the hippoeampal tissue of rats was extracted. The activity of SOD showed： the activity of SOD in Edaravone group was significantly higher than that of the model group （P 〈 0.05） at the time of one month and two month after operations. When compared with Duxil group, the activity of SOD in Edaravone group did not found significantly difference （P 〉 0.05）, while the therapeutic effect at the time of one month was significantly higher than that of two month. The content of MDA in model group was significantly higher than that of the Edaravone group and the Duxil group （P 〈 0.05）. The effect of indexs was better in the two month than those in the one month. [Conclusion] Edaravone could decrease the content of MDA in hippocampal tissue of rat with vascular dementia, and increase the content of SOD. It suggests that edaravone have obvious effect on ehminating the free acids in brain tissues with hypoxia and postischemia.