枸杞多糖对DES诱导的仓鼠睾丸氧化损伤的保护作用

Protection of Lycium Barbarum Polysaccharides on Diethylstilbestrol Induced Oxidative Damage in Testis of Hamster

为研究枸杞多糖(LBP)对己烯雌酚(DES)诱导的仓鼠睾丸氧化损伤的保护作用,将24只成年雄性仓鼠随机分为4组,即正常对照组、DES组、DES+LBP组、DES+VC+VE组。连续处理7 d后统计睾丸质量和相对质量,观察睾丸组织结构的变化,检测睾丸组织和血浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)和丙二醛(MDA)的含量。结果LBP显著逆转了DES引起的生精损害,睾丸质量显著上升(P<0.05),曲细精管排列有序,生精细胞轮廓清晰。同时LBP显著提高体内SOD和T-AOC含量(P<0.01),降低MDA水平(P<0.01)。给予LBP后各项指标均接近对照组,与补充维生素组没有显著性差异。说明枸杞多糖可有效对抗自由基,抑制脂质过氧化,减弱DES导致的生精损害,提示LBP是缓解环境雌激素生殖毒性的有效成分之一。

Protective effects of Lycium Barbarum polysaccharides （LBP） on diethylstilbestrol （DES） induced oxidative damage in hamster testis was studied in this experiment. Adult hamsters （Mesocricetus auratus） were randomly divided into 4 groups： Control group, DES group, DES＋LBP group, DES＋Vc ＋VE group. Absolute and relative testieular weight were calculated, morphological changes of testis were observed and the contents of SOD, GSH-Px, T-AOC, MDA in testis tissue and plasma were detected after 7 successive days treatment. The results showed that LBP had an evident effect in reversing the reproductive damage induced by DES. In DES＋ LBP group, the absolute and relative testieular weight were significantly upgraded （P 〈0.05） and the seminiferous tubules were lined up in order. Levels of SOD and T-AOC elevated markedly in LBP group （P 〈0.01） ,which was similar with control, but MDA showed the reverse tendency. There was no significant difference between LBP and vitamin group. It is concluded that LBP could efficiently restrain the production of reactive oxygen species, inhibit the generation of lipid peroxidation, accordingly attenuate the reproductive toxicity of DES. These results indicated that LBP is one of the active components in attenuating the oxidative damage induced by environmental estrogens.