摘要
目的探讨NF-κB在重症急性胰腺炎各器官中的表达及其介导的细胞因子TNF-α、IL-10表达的变化,并研究茵陈承气汤对NF-κB活性及炎症介质表达的影响。方法采用十二指肠乳头逆行插管注射牛磺胆酸钠法建立大鼠重症急性胰腺炎模型,60只健康SD大鼠随机分为对照组、模型组和中药治疗组,每组20只。采用免疫组织化学法检测胰腺、肺和空肠组织NF-κB的活性和放免法检测TNF-α、IL-10的表达,并取胰腺、肺和空肠组织甲醛固定,石蜡包埋切片,苏木精-依红染色后光学显微镜下观测组织形态学变化。结果NF-κB的活性及TNF-α、IL-10的表达3组中差异明显;中药治疗组与模型组比较,胰腺、肺和空肠组织NF-κB的活性和TNF-α、IL-10的表达水平均明显降低(P<0.05);病理观察:胰腺、肺和空肠组织损伤对照组无明显形态学变化,模型组较重,中药治疗组明显改善。结论NF-κB在重症急性胰腺炎的胰腺病变、肺损伤、空肠损害中起重要作用;茵陈承气汤能有效改善病情,可能与抑制NF-κB的活性有关。
Objective To investigate the effect of Yinchenchengqi decoction for the the activeness of NF-κB and the expression of inflammatory mediators in severe acute pancreatitis models. Methods SD rats were divided at random into the control group, model group and herbal (Yinchenchengqi decoction)group. The NF-κB was detected with immunohistochemistry and the histologial appearance of pancreas,lung and jejunum were observed under microscope. TNF-a, IL-10 were observed by radioimmumoassay. Results The results showed that in the herbal group the activeness of NF-κB in pancreas, lung and jejunum and the serum levels of TNF-a, IL-10 decreased remarkably(P〈0.05). The histology scores results definitively demenstrated the damages of pancreas,lung and jejunum in the model group were more serious than in the control group and the histology score of the herbal group remarkly decreased compared with the model group. Conclusion NF-κB play an important role in the desease of pancreas,lung and jejunum of SAP; The possible reason of that Yinchenchengqi Decoction could improve the conditions of SAP may be it could suppress the activeness of NF-κB and reduce the expression of inflammatory mediators.
出处
《江西医学院学报》
CAS
2008年第2期29-31,共3页
Acta Academiae Medicinae Jiangxi
基金
江西省中医管理局课题(030041)