哮喘患儿血清中可溶性干细胞因子的检测及临床意义

Detection and Significance of S-SCF in Peripheral Blood from Children with Bronchial Asthma

目的探讨支气管哮喘患儿不同病期外周血清中可溶性干细胞因子(soluble stemcell factor,S-SCF)的浓度变化及其临床意义。方法用酶联免疫吸附试验检测支气管哮喘患儿急性发作期(哮喘发作组)、缓解期(哮喘缓期组)及健康儿童(对照组)各30例血清中的S-SCF、β-类胰蛋白酶(β-tryptase)浓度。结果哮喘发作组患儿血清S-SCF和β-类胰蛋白酶水平显著高于哮喘缓解组和对照组(P<0.05);哮喘缓解组患儿血清S-SCF和β-类胰蛋白酶和对照组比较差异无统计学意义(P>0.05)。结论S-SCF可能是支气管哮喘发作机制中的重要因素之一,为临床评价哮喘发作的严重程度及治疗提供了一条新的途径。

Objective To investigate the density and clinical significance of S-SCF and β-tryptase in peripheral blood from children with different stage of bronchial asthma. Methods Serum S-SCF and β-tryptase were measured by enzyme linked immunosorbent assay in 30 children with acute asthmatic attack, 30 children with asthmatic remission and 30 healthy controls. Results The levels of S-SCF and β-tryptase in the children with asthmatic acute attack were significantly higher compared with the children with asthmatic remission and the control group. The levels of S-SCF and β-tryptase in the children with asthmatic remission were nonsignificant compared with the control group（P〉0.05）. Conclusion S-SCF may play a key role in mechanisms of asthma in children and provide a new way in treatment and clinical evaluation in children with asthma.