慢性阻塞性肺疾病患者外周血IL-8的测定及其意义

Detection and Significance of Serum Interleukin-8 in Chronic Obstructive Pulmonary Disease

目的探讨IL-8在慢性阻塞性肺疾病(COPD)发病机制中的作用。方法采用酶联免疫吸附法(ELISA)检测30例COPD患者(急性加重期及治疗后稳定期)和10例健康体验者(对照组)外周血IL-8浓度,并测定第1秒最大呼气量占预计值百分比(FEV1%)、中性粒细胞百分比。结果COPD急性加重期组外周血IL-8水平和中性粒细胞百分比明显高于治疗后稳定期组和对照组(P<0.05)。治疗后稳定期组IL-8亦显著高于对照组(P<0.05)。COPD急性加重期与治疗后稳定期外周血IL-8与FEV1%呈负相关(r=-0.97,P<0.01;r=-0.77,P<0.01)。结论IL-8参与COPD气道炎症的形成,并与气道阻塞程度密切相关。

Objective To evaluate the role of interleukin-8 in the pathogenesis of COPD. Methods The serum levels of IL-8 were evaluated by ELISA method from 30 patients with acute exacerbation of COPD and the patients after therapy to stable phase and 10 healthy control subjects. Also their pulmonary function and the percentage of neutrophils were evaluated. Results The percentage of neutrophils and the level of IL-8 in serum from acute exacerbation of COPD patients were significantly higher than those from stable COPD patiens and from healthy control subjects（P〈0.05）. FEV1 % correlated negatively with IL-8 from acute exacerbation of COPD and stable COPD（r= -0.96, P〈0.01,r=-0.77,P〈0.01）. Conclusion IL-8 is involved in airway inflammation witch results in airflow obstruction. IL-8 is an important role in COPD patiens.