绿原酸体外抗单纯疱疹病毒作用

Anti-herpes Simplex Virus effect of Chlorogenic Acid in vitro

目的:研究绿原酸对单纯疱疹病毒I型(HSV-1)的体外抑制作用。方法:采用细胞培养技术,通过绿原酸预处理对病毒的抑制作用、病毒感染细胞同时使用绿原酸和预先使用绿原酸再感染病毒3种给药方法,利用生物显微镜观察幼仓鼠肾细胞(BHK-21)发生病变的情况及观察实验组与病毒对照组病毒感染的滴度。结果:绿原酸在BHK-21细胞上具有明显的抗病毒作用,绿原酸对病毒的最小有效浓度(MIC)为1.0μg.mL-1,而且毒性低,最大无毒浓度(TD0)为200μg.mL-1,其治疗指数(TI)为200;绿原酸浓度1μg.mL-1预处理细胞24h以上能有效抑制病毒感染;绿原酸对HSV-1病毒无直接杀伤作用。结论:绿原酸在体外实验中对HSV-1感染具有明显的抗病毒作用,且能有效预防病毒感染的作用,但对病毒无直接杀死作用。

AIM： To study the antiviral activity and mechanism of Chlorogenic Acid and develop a safe and effective antiviral agent against Herpes Simplex Virus（HSV-1）. METHODS： The cytopathic effects （CPE）of CHA on BHK-21 cells was observed. The directly killing effect, inhibitory effect and interdictory effect were tested respectively under the microscope by three ways： drug used directly, drug used while cell were infected and drug used prior to infection. RESULTS： In cell culture, the drug was found to be a inhibitor of HSV-1 in a concentration-dependent manner. The minimal inhibitory concentration （MIC） was 1 μg·mL^-1, the maximal innocuous dose （TD0） was 200 μg·mL^-1, and the treatment index （TI） was 200. The CHA showed remarkable inhibitory effects on HSV-1. Also, CHA showed significant interdictory effects on virus infection pretreated with CHA above the concentration 1 μg·mL^-1; CHA have no effective on killing Virus directly. CONCLUSION： CHA has antiviral activity on HSV-1 in vitro, and remarkable prevent virus infection, but have no directly killing effect.