脐血血管内皮祖细胞移植对心肌梗死血管形成的影响

Effects of umbilical cord blood endothelial progenitor cells transplantation on angiogenesis after myocardial infarction

目的探讨犬脐血血管内皮祖细胞(EPCs)移植对心肌梗死血管形成的影响。方法取妊娠犬脐血,体外分离、培养、扩增EPCs,免疫组化鉴定。建立成年杂种犬梗死模型,经BrdU标记的EPCs灌注移植入梗死区域,1、4、8周后处死动物,取心肌标本HE染色确认梗死模型、免疫组化染色BrdU观察EPCs参与梗死心肌血管形成、vW因子染色观察梗死心肌血管并计数以观察EPCs移植组与对照组血管形成差异。结果免疫组化检测结果表明培养的细胞为EPCs;HE染色示心肌梗死区有大量瘢痕组织、成纤维细胞及小血管形成;免疫组化检测显示梗死心肌区域标本内小血管上存在BrdU阳性细胞;EPCs移植组与对照组心肌梗死后第1、4、8周的心肌缺血区和梗死区的血管计数均无显著差异。结论犬脐血EPCs梗死心肌移植可参与血管形成,但不能促进心肌的血管再生。

Objective To investigate the effects of canine umbilical cord blood endothelial progenitor cells （EPCs） transplantation on angiogenesis after myocardial infarction. Methods Umbilical cord blood was obtained from pregnant canine, and EPCs were isolated, cultured, expanded and identified by immunohistochemistry. EPCs, labeled by BrdU, were infused through the coronary artery into the canine model of myocardial infarction. The animals were sacrificed after 1, 4, 8 weeks, and specimens were observed by HE staining in confirmation of the myocardial infarction model. BrdU was detected by immunohistochemical staining to confirm the participation of EPCs in the angiogenesis, and vW factor was observed and blood vessels were counted to compare the difference of angiogenesis between the EPCs transplantation group and control group. Results It was demonstrated by immunohistochemistry that the cultured cells were EPCs. Scar tissues, fibroblasts, and angiogenesis were observed by HE staining in the infarction area. The BrdU positive cells were detected by immunohistochemistry on the vessels of the infarction area. However, there was no significant difference in the counting of blood vessels between the EPCs transplantation group and control group. Conclusion Canine umbilical cord blood EPCs transplantation participate the formation of blood vessels, but can not promote the angiogenesis after myocardial infarction.