三叶青提取物对人结肠癌细胞系RKO细胞凋亡的影响

Influence of Clover Extract on Human Colon Cancer Cellular PKO Apoptosis

[目的]观察三叶青提取物对人结肠癌细胞系RKO细胞凋亡的作用,初步探讨其可能的机制。[方法]采用体外细胞培养技术,用MTT法观察三叶青提取物RKO细胞的影响;琼脂糖凝胶电泳检测细胞凋亡;RT-PCR检测Bid,Bax,Bcl-2和Bcl-XL的表达改变。[结果]三叶青提取物能显著地抑制人结肠癌细胞系RKO细胞的生长,并且具有浓度依赖性,且细胞有明显的凋亡特征性改变,并能下调Bcl-XL、上调Bid来诱导凋亡。[结论]三叶青提取物具有对人结肠癌细胞系RKO细胞抗增殖和诱导凋亡的作用,且呈剂量依赖性。

[Objective] To observe clover extract＇s effect on human colon cancer PKO cellular apoptosis, primarily explore its possible mechanism. [Method] Take cell culture in vitro and MTT technology to observe clover extract＇s influence on PKO cell, test apoptosis with agarose gel electrophoresis, test the changes of Bid, Bax, Bcl-2 and Bcl-XL with PT-PCR. [Result] Clover extract can markedly inhibit PKO growth, with dependence of concentration and obvious apoptosis change, also can down-regulate Bcl-XL, up-regulate Bid to cause apoptosis. [Conclusion] Clover extract has anti-hyperplasia and induces apoptosis to human colon cancer cellular PKO, in dependence of concentration.