摘要
[目的]建立生首乌和制首乌中大黄素含量的测定方法。[方法]采用HPLC法,SPHERISORB ODS2(150×4.6mm,5μm)色谱柱,流动相为甲醇-0.1%磷酸(85:15),检测波长为254nm,柱温28℃,流速1.0ml.min^-1。[结果]回归方程为Y=3352.3X+37.297,线性范围为0.03~0.20μg。生首乌的平均回收率分别为96.6%(RSD=1.3%)。[结论]本方法准确灵敏,重复性好,可用于何首乌药材的质量控制。
[Objective]Make measurement of emodin content in raw and prepared fleece-flower root. [Method]Take HPLC and the colorspectrum column SPHERISORB ODS2 (150 × 4. 6mm, 5μm), the flow spectrum being methanol - 0. 1 % phosphate(85:15), the measurement wave length: 254nm, column temperature: 28℃, flow speed.. 1.0 ml · in^-1. [Result] The regression equation Y= 3352.3X+ 37. 297,the linear range:0. 03-0.20μg, the average reception rate of the raw fleece-flower root:96.6 % (RSD= 1.3%). [Conclusion]The said method is correct and quick, with good repeat, can be used for quality control of fleece-flower root drug.
出处
《浙江中医药大学学报》
CAS
2008年第3期399-400,共2页
Journal of Zhejiang Chinese Medical University