肠杆菌CB-2的生长特性及质粒特性

Growth and plasmid characteristics of enterobacter sp.CB-2

为研究微生物对氯苯的降解特性和生长特性,采用从活性污泥样品中分离得到的一株肠杆菌CB-2对氯苯进行降解实验.结果表明:肠杆菌CB-2在氯苯质量浓度为100mg/L的无机盐液体培养基中,24 h内对氯苯的降解率可达81.2%;肠杆菌CB-2不经诱导抗氯化汞的能力达20 mg/L;可以耐受的氯苯质量浓度为500 mg/L,并具有较宽的底物利用范围.为确定降解基因的位置,采用苯甲酸钠法对肠杆菌CB-2进行质粒消除.实验结果表明,质粒与氯苯的降解性有关,而与抗生素的抗性无关.

An enterobacter sp. CB- 2 isolated from the activated sludge samples was used for microbial degradation experiment on chlorobenzene to research characteristics of microbial degradation and growth in chlorobenzene. Experimental results show that enterobacter sp. CB- 2 could degrade chlorobenzene in mineral medium containing 100 mg/L chlorobenzene, with degradation efficiency reaching 81.2% within 24 hours. Enterobacter sp. CB - 2 has high resistance to mercuric chloride, which the highest resistance without induction is 20 mg/L of mercuric chloride.Enterobacter sp. CB-2 is well-tolerated in chlorobenzene with 500 mg/L, and it can also be used to degrade some other substrates. Moreover, to confirm the location of degradation gene, plasmid elimination of enterobacter sp. CB- 2 was performed with sodium benzoate. Experiment results confirm that the plasmid is related to the characteristic of chlorobenzene degradation, but not to antibiotic resistance.