荔枝核对小鼠S180、EAC肿瘤细胞Bax和Bcl-2蛋白表达的影响研究

Effects of Semen Litchi on the Expressions of S180 and EAC Tumor Cells and Bax and Bcl-2 Proteins in Rats

目的:研究荔枝核对小鼠S180、EAC肿瘤细胞Bax和Bcl-2蛋白表达的影响。方法:采用小鼠腋下接种瘤株的方法,复制EAC、S180及肝癌荷瘤动物模型。小鼠EAC、S180细胞与肝癌细胞混悬液分别用生理盐水按1∶1进行稀释,制成含瘤腹水混悬液。小鼠在给药前24h进行腋窝皮下接种,每只0·2mL,整个操作在无菌条件下60min内完成。连续给药10d,观察EAC、S180肿瘤和肝癌肿瘤细胞的生长情况。结果:荔枝核能够显著抑制EAC、S180肿瘤和肝癌肿瘤细胞的生长。结论:荔枝核具有抑制小鼠EAC、S180、肝癌细胞的作用;具有抑制小鼠S180、EAC体内、外细胞生长的作用;并能促进肿瘤细胞凋亡,从而发挥抗肿瘤的作用。

OBJECTIVE： To study the effects of Semen Litchi on the expressions of S180 and EAC tumor cells and Bax and Bcl - 2 proteins in rats. METHODS： EAC, S180 and hepatic carcinoma tumor - bearing mice models were established in mice by inoculating them with tumor strains, EAC, S180 and hepatic carcinoma suspensions were diluted respectively with normal saline in a ratio of 1 ： 1 for the preparation of tumor- bearing ascites suspension. Each rat was subcutaneously inoculated with the mixture 0,2 mL via axilla 24 hours before medication, with the whole sterile operation finished within 60 minutes. After treated with Semen Litchi for 10 consecutive days, the growth of EAC, S180 and hepatic carcinoma in mice was observed, RESULTS： The growth of EAC and S180 and hepatic carcinoma cells in mice was significantly inhibited by Semen Litchi. CONCLUSION： Semen Litchi can inhibit the growth of S180 and EAC tumor cells in vitro and in vivo, and its antitumor effect is related to its ability to promote apoptotic of tumor cells.