正交试验优选盐酸小檗碱固体脂质纳米粒制备工艺

Optimization of the Preparation Technology of Berberine Hydrochloride Solid Lipid Nanoparticles by Orthogonal Experiment

目的:制备盐酸小檗碱固体脂质纳米粒(BH-SLN)。方法:用旋转蒸发薄膜超声法制备BH-SLN;在单因素考察的基础上,通过正交试验优选该纳米粒的处方及制备工艺,并对优化条件下制备的BH-SLN进行质量评价。结果:制备的BH-SLN大小较均匀,平均粒径为60·5nm,Zeta电位为—29·7mV,载药量为8·69%,包封率为97·58%。结论:本优化工艺制备得到的BH-SLN包封率高、稳定性好,方法可靠。

OBJECTIVE： To prepare Berberine hydrochloride solid lipid nanoparticles（BH- SLN）. METHODS： BH- SLN was prepared by conventional rotary-evaporated film- ultrasonication method. Based on the single- factor experiment, BH- SLN was prepared using orthogonal design for optimization of formulation and technology. The quality of the obtained BH- SLN was evaluated. RESULTS： BH- SLN had even and regular appearance with a mean diameter of 60.5 nm, Zeta potential of -29.7 mV, drug loading of 8.69%, and entrapment ratio of 97.58%. CONCLUSION： The entrapment ratio of BH- SLN is high and the stability is good for BH - SLN prepared by conventional rotary - evaporated film - ultrasonication method, and the method is reliable.