陈香舒胃散质量标准研究

Quality Standard of Chenxiang Shuwei Powder

目的：建立陈香舒胃散的质量标准。方法：采用薄层色谱（TLC）法对方中大黄进行定性鉴别；采用反相高效液相色谱法对橙皮苷进行定量分析：色谱柱为LunaC18（2）（150mm×4．6mm，5μm），流动相为甲醇-冰醋酸-水（35：4：61），流速为1．0mL·min^-1，检测波长为283nm。结果：TLC斑点清晰，分离度较好。橙皮苷进样量在0．12-2．40μg范围内与峰面积积分值呈良好线性关系（r=0．9999）；平均加样回收率为98．0％，RSD=0．9％（n=9）。结论：所建标准可用于陈香舒胃散的质量控制。

OBJECTIVE： To establish the quality standard of Chenxiang shuwei poweder. METHODS： The qualitative analysis of Rhubarb in Chenxiang shuwei powder was carried out using TLC and the quantitative analysis of Hesperidin was carried out using RP- HPLC on a column of Luna C18（2） （150 mm · 4.6 mm, 5μm） . The mobile phase consisted of methyl alcohol - acetic acid - aquifer（35 ： 4 ： 61） with a flow rate of 1,0 mL · min ^-1 at a detection wavelength of 283 nm. RESULTS： The TLC spots of Chenxiang shuwei powder were clear with good resolution. The linear range of Hesperidin was 0.12-2.40 μg （r =0.999 9） . The average recovery was 98.0% （RSD = 0.9%, n = 9） ,CONCLUSION： The established standard is applicable for the quality control of Chenxiang shuwei powder.