分光光度法测定鬼针草提取物中总黄酮的含量

Determination of Total Flavonoids in Extract of Bidens bipinnata L. by Spectrophotometry

目的：建立以分光光度法测定鬼针草提取物中总黄酮含量的方法。方法：以乙醇提取药材、HPD100大孔吸附树脂纯化，以芦丁为对照品，于510nm波长处测定鬼针草提取物中总黄酮的含量。结果：芦丁检测浓度在0．00816～0．04896mg·mL^-1范围内与吸光度呈良好线性关系（r=0．9993）；平均加样回收率为99．03％，RSD=2．27％（n=6）。结论：本法灵敏度高、稳定性好，可用于鬼针草的质量控制。

OBJECTIVE： To determine the content of total flavonoids in extract of Bidens bipinnata L. by spectrophotometry. METHODS： The Bidens bipinnata L. was extracted with ethanol and purified by HPD100 macroporous resin. With rutin as reference substance, the content of total flavonoids in the extract of Bidens bipinnata L. was determined by spectrophotometry at a wavelength of 510 mn. RESULTS： The linear range of Rutin was 0.008 16-0.048 96 mg · mL ^-1（r = 0.999 3）, its average recovery was 99.03% （RSD = 2.27%, n =6） .CONCLUSION： The method is of high sensitivity and good stability, and it is applicable for the quality control of Bidens bipinnata L.