白斑膏质量标准研究

Quality Standard of Baiban Paste

目的：建立白斑膏的质量标准。方法：采用薄层色谱（TLC）法对白斑膏中补骨脂、红花2种中药进行定性鉴别；采用高效液相色谱法测定补骨脂素和异补骨脂素的含量。结果：补骨脂、红花的TLC特征斑点清晰，分离度好，阴性无干扰；补骨脂素、异补骨脂素进样量分别在13．44－67．20ng（r=0．99926）、12．00-60．00ng（r=0．99929）范围内与各自峰面积积分值呈良好线性关系；平均加样回收率分别为98．86％、98．84％，RSD分别为1．09％、1．29％（n=6）。结论：所建标准可用于白斑膏的质量控制。

OBJECTIVE： To establish the quality standard for Baiban paste. METHODS： The two component - Fructus Psoralen and Flos Carthami in Baiban paste were identified by TLC. The contents of Psoralen and Isopsoralen in Baiban paste were determined by HPLC. RESULTS： The TLC spots of both Fructus Psoralen and Flos Carthami were clear with good resolution and free of interference of negative samples. The linear ranges of Psoralen and Isopsorale wre 13,44-67.20 ng and 12.00-60.00 ng, respectively. The average recoveries were 98.86% （RSD = 1.09%, n = 6） and 98.84% （RSD = 1.29%, n = 6）, respectively. CONCLUSION： The established standard is applicable for the quality control of Baiban paste.