摘要
为探讨As2O3诱导MD肿瘤细胞株凋亡的机制,采用MTT法检测As2O3对MDCC-MSB1的抑制作用,采用荧光显微镜观察细胞形态学变化,DNA Ladder法检测细胞凋亡,Fura-2/AM负载双波长荧光分光光度计检测细胞内游离钙离子浓度([Ca2+]i)变化,观察As2O3对鸡MD肿瘤细胞株MDCC-MSB1的影响。结果显示:As2O3能抑制鸡MD肿瘤细胞株MDCC-MSB1的生长,呈剂量依赖关系(P<0.05或P<0.01);在荧光显微镜下可见As2O3作用后MDCC-MSB1细胞呈现典型的凋亡特征,并出现典型的DNA Ladder,双波长荧光分光光度计检测结果显示:As2O3作用后的MDCC-MSB1细胞内[Ca2+]i升高(P<0.05或P<0.01),呈剂量依赖关系。As2O3能明显抑制鸡MD肿瘤细胞株MDCC-MSB1细胞的生长,并诱导其发生凋亡,细胞内[Ca2+]i升高可能是其诱导细胞凋亡的机制之一。
To explore the mechanism of MDCC-MSB1 apoptosis induced by Arsenic Trioxide （As2Os）,inhibition percentage was detected by MTT assay;morphology changes was examined by fluorescence microscope;apoptosis was examined by DNA Ladder; [Ca^2＋]i was investigated by spectrofluorimeter on MDCC-MSB1 cells. The results were follows： As2O3 inhibited the proliferation of MDCC-MSB1 cells in concentration dependent manner （P〈0.05 or P〈0.01）; typical apoptosis character was seen by fluorescence microscope;DNA Ladder were observed;spectrofluorimeter analysis showed that the [Ca^2＋]i was elevated significantly after the treatment of As2O3 （P〈0.05 or P〈0.01）and showed relationship of concentration dependent. As2Os can induce MDCC-MSB1 apoptosis. The increase of [Ca^2＋]i may be one of its mechanisms.
出处
《中国家禽》
北大核心
2008年第10期21-24,共4页
China Poultry
基金
国家自然科学基金资助项目(30170711)
