多孔磷酸钙骨水泥组织工程支架的高分子灌注增强

Reinforcement of macroporous calcium phosphate cement scaffold by polymers infiltration

利用棒状谷氨酸钠晶体作为造孔粒子,采用可溶盐造孔法,制备了三维连通的大孔径多孔磷酸钙骨水泥支架,分别将明胶(Gelatin)、聚乳酸-羟基乙酸共聚物(PLGA)、聚乳酸(PLA)、聚己内酯(PCL)、聚羟基丁酸戊酸酯(PHBV)灌注到多孔磷酸钙骨水泥(CPC)支架的孔隙中以改善支架材料的力学性能。结果表明,5种高分子材料与水的接触角大小顺序为PHBV>PCL>PLA>PLGA>Gelatin,复合支架材料的强度随高分子材料与水接触角的减小而增大;除PHBV外,其余4种均有明显的增强效果,其中Gelatin/CPC复合支架增强效果最好,强度达到2.25 MPa±0.02 MPa,是CPC支架强度的25倍。经过增强的大孔径多孔磷酸钙骨水泥复合支架可用作骨组织工程支架材料。

The macroporous calcium phosphate cement （CPC） scaffold was fabricated by dissolving the rod-shaped monosodium glutamate crystals. Gelatin, PLA, PCL, PLGA and PHBV solutions were infiltrated into the pores of a CPC scaffold to improve its mechanical strength. The results indicate that the contact angle of water on the polymers varies from each other in the sequence of PHBV 〉 PCL〉 PLA〉 PLGA〉 Gelatin, and the compressive strength of the composite scaffolds increases with the increase of the hydrophilicity of the polymers. The CPC scaffold is markedly reinforced by the polymers infiltration except for PHBV, and the strength of Gelatin/CPC scaffold reaches 2.25 MPa, which is 25 times that of CPC scaffold. The reinforced CPC scaffolds are promising for being used in the bone tissue engineering.