金黄地鼠腹侧前列腺在体修饰精子膜蛋白作用研究

Ventral Prostate Glands Modification of Sperm Membrane Proteins in Golden Hamster

【目的】在整体水平探讨前列腺分泌蛋白对金黄地鼠精子膜蛋白的影响。【方法】雄性鼠手术分为4组,分别为附属性腺全去组,腹前列腺摘除组,去除其他附属性腺仅保留腹前列腺组和假手术组,每组动物6只。收集与保留前列腺及去除组的雄性鼠交配后的子宫腔精子,抽提精子膜蛋白,膜蛋白经聚丙烯酰胺凝胶(SDS-PAGE)和二维电泳凝胶分析,比较前列腺存在与否精子膜蛋白组分的异同。【结果】与不同组雄鼠交配后收集的子宫腔精子膜蛋白SDS-PAGE电泳谱主要条带类似,含腹前列腺组的精子膜组分中相对分子质量15k、29k、38k、55k和91k多肽的量较去除腹前列腺组的子宫腔精子膜相应组分增加。二维电泳结果示与含腹前列腺组雄鼠交配后收集的子宫腔精子膜蛋白较无腹前列腺组的精子膜蛋白多出10个蛋白斑点(MM/IP分别为16k/8.60、16.5k/9.2、28k/5.88/6.10、29k/5.98、32k/6.35/6.50/7.20、61k/5.90、83k/6.40),另外蛋白斑点量增加有11个。【结论】腹前列腺可修饰和调节精子膜蛋白,进而这些蛋白可能对雄性生殖力及胚胎的发育起作用。

[Objective] To investigate the effect of the secretory proteins of the ventral prostate glands on the sperm membrane proteins in golden hamsters. [Methods] The sperm was collected from female hamsters uteri after mated with the males with or without ventral prostate gland. Male golden hamsters were divided into four experimental groups： （i） all accessory sex glands （ASG） removed ; （ii） ventral prostate gland removed ; （iii） all ASG removed except ventral prostate gland and （iv） sham-operated controls. Each group contained 6 hamsters. The sperm membrane proteins were extracted from uterine sperm and analyzed by SDS-PAGE and two dimension electrophoresis. [Results] The SDS-PAGE results of sperm membrane showed that the ventral prostate glands added up some proteins or increased the quantity of some proteins, which contained molecular mass （MM）15 k, 29 k, 38 k, 55 k, and 91 k proteins, to the sperm membrane. 2D-electrophoresis of sperm membrane showed that some extra protein spots were appeared in with ventral prostate gland groups, their MM and isoelectric point （IP） corresponding to 16 k/8.60, 16.6 k/9.20, 28 k/5.88, 28 k/6.10, 29 k/5.98, 32 k/6.35, 32 k/6.50, 32 k/7.20, 61 k/5.90,and 83 k/6.40, respectively. The quantity of some protein spots increased in sperm membrane of ventral prostate glands group, their MM/IP coresponding to 17 k/5.95, 17.5 k/6.50, 24 k/7.20, 26 k/5.40, 26 k/ 5.60, 27 k/7.20, 27 k/7.50, 28 k/5.70, 29 k/8.50, 42 k/6.50, and 42 k/7.00, respectively. [Conclusion] The ventral prostate gland secretion plays a role in regulating the sperm membrane proteins and the latter may in turn affect the male fertility or embryo development.