脂肪组织中IRS-1相关的PI3K活性在多囊巢综合征胰岛素抵抗中的作用

Insulin Receptor Substrate I-Associated Phosphatidylinositol 3-Kinase (PI3K) Activity in Adipose Tissue of PCOS

【目的】探讨多囊卵巢综合征(PCOS)脂肪组织胰岛素受体底物I相关的磷脂酰肌醇3激酶(IRS-1-Associcrted-PI3K)的活性在PCOS发病中的作用。【方法】PCOS患者和对照者根据体质量指数(BMI)分为PCOS肥胖组、PCOS非肥胖组、肥胖对照组和非肥胖对照组,各12例,采用免疫沉淀、薄层层析、放射自显影半定量检测脂肪组织IRS-1相关的PI3K活性。【结果】脂肪组织中IRS-1-Associated PI3K活性在PCOS肥胖组为55%±24%,较非肥胖对照组84%±16%明显降低(P<0.001);肥胖对照组为46%±22%,PCOS非肥胖组为71%±26%,均较非肥胖对照组明显降低(P<0.001和P<0.05),PCOS肥胖组和肥胖对照组之间以及PCOS肥胖组和PCOS非肥胖组之间的差异均无显著性意义(P>0.05)。【结论】PCOS肥胖组和PCOS非肥胖组脂肪组织IRS-1相关的PI3K活性均较非肥胖对照组明显减弱,可能抑制胰岛素受体后的信号传导,并与PCOS胰岛素抵抗的发生有关。

[Objective] To study insulin receptor substrate Ⅰ-associated phosphatidylinositol 3-kinase （PI3K） activity of adipose tissue in polycystic ovary syndrome （PCOS） to approach the mechanism of insulin resistance （IR） in PCOS at the tissular and cellular level. [Method] Patients with PCOS and healthy controls were divided into the obese PCOS group, the non-obese PCOS group, the obese control group and the non-obese control group according to their body mass index （BMI） and with 25 cases each group. PI3K activity of adipose tissue were studied with immunoprecipitation, TLC, and phosphorimaging. [Result] PI3K activity of adipose tissue in the obese PCOS group （55% ± 24%, P 〈 0.001）, the obese control group （46% ± 22%, P 〈 0.001） and in the non obese PCOS group （71% ±26%, P 〈 0.05） were markedly lower than the non obese control group（84%± 16%）. The difference both between the obese PCOS group and the obese control group and the two PCOS groups were not significant （P 〉 0.05）. [Conclusion] PI3K activity of adipose tissue in the two PCOS groups both decrease markedly than the non obese control group. It might lead to the disorder of insulin-signaling transduction downstream of insulin receptor and concern with the IR.