Caspase-3与Caspase-6蛋白在肾透明细胞癌中的表达及临床意义

Expression and clinical significance of Caspase-3 and Caspase-6 proteins in RCCC

目的探讨Caspase-3、Caspase-6蛋白在肾透明细胞癌(RCCC)的表达及与预后的关系。方法采用免疫组化(SP法)对75例RCCC及其癌旁组织、15例正常肾组织标本中的Caspase-3、Caspase-6蛋白的表达进行研究,分析与RCCC临床病理特征及其术后生存率的关系。结果Caspase-3蛋白在RCCC中阳性表达率低于癌旁对照组,差异有显著性(P<0.01);在病理分级中的阳性表达率G3低于G1;在临床分期中的阳性表达率Ⅲ～Ⅳ期低于Ⅰ期;在淋巴结转移阳性组中阳性表达率低于阴性组,差异均有显著性(P<0.05)。Caspase-6蛋白在RCCC中阳性表达率低于癌旁对照组,差异有显著性(P<0.05);在不同病理分级和临床分期中阳性表达率差异均无显著性(P>0.05);在淋巴结转移阳性、阴性组中的阳性表达率差异无显著性(P>0.05)。在RCCC中,统计学显示Caspase-3与Caspase-6蛋白表达无相关性(C=0.0921,P>0.05)。在35例随访患者中,Caspase-3、Caspase-6蛋白表达阳性、阴性的术后3年死亡率差异均无显著性(P>0.05)。结论Caspase-3蛋白在RCCC中的阳性表达率低于癌旁和正常肾组织,与临床分期、病理分级呈负相关,其低表达与淋巴转移有关。Caspase-6蛋白在RCCC中的阳性表达率低于癌旁和正常肾组织,但与临床分期、病理分级及淋巴转移无关。在RCCC中,Caspase-3蛋白的表达与Caspase-6蛋白的表达无相关性。

[ Objective ] To investigate the expression and prognosis of Caspase-3 and Caspase-6 proteins in human renal clear cell carcinoma（RCCC）. [Method] 75 RCCC and their para-tumor tissues and 15 normal renal tissues were analyzed for expression of Caspase-3 and Caspase-6 proteins by SP immunohischemistry and correlation with thecharacters and prognosis of RCCC. [Result] The positive rate of Caspase-3 protein in all the RCCC patients was significantly lower than that in para-tumor tissue （P 〈0.01）. In G1,G2,G3 of RCCC , the difference of Caspase-3 protein positive rate only between G1 and G3 （P 〈0.05） .In Ⅰ,Ⅱ , Ⅲ-Ⅳ of clinical stage , the difference of Caspase-3 protein positive rate only between Ⅰ and Ⅲ-Ⅳ （P 〈0.05）. The difference between the positive rate and the negative rate of Caspase-3 protein in that with lymph nodes metastasis was significantly （P 〈 0.05）. The positive rate of Caspase-6 protein in all the RCCC patients was lower than that in para-tumor tissue （P 〈 0.05）. In G1,G2,G3 of RCCC, the difference among them was not significant（P 〉0.05）. The difference between the positive rate and the negative rate of Caspase-6 protein in that with lymph nodes metastasis was not significant （P 〉 0.05）. In RCCC tissue, the expression of Caspase-3 and Caspase-6 proteins may not correlate with each other.After 3 years follow-up in 35 cases, The difference between the mortality rate in 3 years of the positive and negative expression of Caspase-3 and Caspase-6 protein was not significant （C=0.0921,P 〉0.05）. [Conclusion] The positive expression of Caspase-3 protein in RCCC was significantly lower than that in para-tumor tissue and normal renal tissue. It may be correlated with the clinical stage and pathologic grade of RCCC and with lymph nodes metastasis in RCCC. The positive expression of Caspase-6 protein in RCCC was significantly lower than that in para-tumor tissue and normal renal tissue. It may not be correlated with the clinical stage and pathologic grade of RCCC and with lymph nodes metastasis. In RCCC, the lower positive expressions of Caspase-3 and Caspase-6 proteins were independent from each other.