大鼠局灶性脑缺血-再灌注后脑组织一氧化氮水平的实验研究

Experimental research of Nitric Oxide level in cerebral ischemia-reperfusion injury of rats

目的本实验通过观察大鼠局灶性脑缺血-再灌注后脑组织一氧化氮水平的变化,以进一步探讨局灶性脑缺血-再灌注损伤机制。方法将雄性成年Wistar大鼠随机分为两组:即假手术组、缺血-再灌注组。每组均观察手术后6h、12h、24h3个时间点的改变。采用线栓法制成大脑中动脉闭塞模型。测定手术后不同时点缺血脑组织一氧化氮的水平。结果缺血-再灌注组各时点一氧化氮水平与假手术组相应时点比较明显升高,差异具有显著性(P<0.05)。结论脑缺血-再灌注后,缺血大鼠脑组织内的一氧化氮水平升高,说明一氧化氮参与了脑缺血-再灌注损伤的病理生理过程。

[Objective] To explore the change of Nitric Oxide level in experimental cerebral ischernia-reperfusion injury of rats and the possible mechanism. [Methods] The model of focal brain isehemia was produced by an intraluminal suture occlusion of the middle cerebral artery （MCA） in rats. 30 adult male Wistar rats were used. The rats were randomly divided into two groups： sham operation group undergoing all surgical procedures but the intraluminal suture occlusion of the middle cerebral artery, isehemia-reperfusion group. Each group was divided into 3 various stages： 6 h, 12 h, 24 h after operation. The levels of Nitric Oxide （NO） in isehemic cerebral tissues were analyzed by speetrophotometry. [Results] The levels of NO at various stages in isehemia-reperfusion group were obviously higher than those in sham operation group （P 〈0.05）. [Conclusion] The levels of NO in isehemic cerebral tissues of rats are notably increased shows that NO attends the pathophysiologieal process of isehemia-reperfusion injury.