参力保对家兔心肌缺血再灌注损伤的保护作用

PROTECTION OF SHENLIBAO AGAINST MYOCARDIAL ISCHEMIA REPERFUSION INJURY IN RABBITS

目的观察参力保对家兔心肌缺血再灌注损伤的保护作用。方法结扎兔冠状动脉左前降支（LAD）30min，再灌注120min后制备家兔心肌缺血再灌注模型。56只家兔，随机分为7组，生理盐水对照组：假手术组，模型组（给予溶媒8．58mg／kg），参力保高剂量组（22．36mg／kg，内含5．2mg／kg人参皂苷Rg2），参力保中剂量组（11．18mg／kg，内含2．6mg／kg人参皂苷Rg2），参力保低剂量组（5．59mg／kg，内含1．3mg／kg人参皂苷Rg2），阳性对照组（给予盐酸尼卡地平注射液），测定血浆磷酸激酸同工酶（CK—MB）、乳酸脱氢酶（LDH）、天门冬氨酸转氨酶（AST）和心肌组织中超氧化物歧化酶（SOD）活性、丙二醛（MDA）及游离脂肪酸（NFFA）含量；高效液相法测定心肌组织去甲肾上腺素（NE）含量。结果参力保能显著降低血浆CK—MB和LDH、AST的活性，与模型组相比有显著差异（F=13．031～32．604，P〈0．05、0．01）。参力保3个剂量组与模型组比较可明显降低心肌MDA含量，提高SOD活性和NE的含量（F=2．880～15．927，q=5．249～12．510，P〈0．05）；参力保高、中、低剂量组NFFA与模型组比较，差异有显著性（Z1=Z2=Z3=-2．329，P〈0．05）。结论参力保对家兔心肌缺血再灌注损伤有保护作用，其机制与清除自由基抑制脂质过氧化反应有关。

Objective To investigate the effect of Shenlibao against myocardial ischemia reperfusion injury in rabbits. Methods An animal model was established by ligation of left desending coronary artery for 30 min and reperfusion for 120 rain. Fifty six rabbbits were randomly divided into seven groups： saline control group, sham-operation group, media control group, high-dose Shenlibao group, moderate-dose Shenlibao group, low-dose Shenlibao group and nicardipine hydrochloride treatment group. Serum level of CK MB and lactate dehydrogenase （LDH）, superoxide dismutase （SOD）, malondialdehyde （MDA） and free fatty acids （NFFA） were detected. Results Compared with the control groups, Shenlibao decreased the activity of CK-MB and LDH in blood plasma （F= 13. 031-32. 604,P〈0. 05,0.01）. Furthermore, Shenlibao of three different-dosage groups increased the activity of superoxide dismutase （SOD） （F= 8. 765, P〈0.01）, reduced malondialdehyde （MDA） （F= 2. 880, P〈0.05） and free fatty acids （NFFA） content in myocardium （Z1 = Z2 = Z3 = - 2. 329, P〈 0.01 ）, and raised myocardial norepinephrine （NE） contents （F=15. 927,P〈0.01）. Conclusion Shenlibao has a protective effect on myocardial ischemia reperfusion injury. The mechanism is related to the clearance of free radicals and inhibition of subsequent lipid peroxidation.