摘要
目的探讨降钙素基因相关肽（CGRP）对HaCaT细胞增殖、凋亡的影响。方法以不同浓度（10^-7～10^-4mmol／L）CGRP作用于HaCaT细胞，用MTT法检测其对细胞吸光度（A）及生长曲线的影响；流式细胞术分析其对细胞凋亡率的影响。结果不同浓度CGRP组A值均较对照组升高（F=7．78，q=3．53～7．71，P〈0．05）。CGRP浓度在10^-7～10^-5mmol／L时HaCaT细胞吸光度随浓度增加而升高，但差异无统计学意义（P〉0．05），在10^-4mmol／L时A值较10^-5mmol／L时反而降低（q=4．18，P〈0．05）。细胞凋亡率随CGRP浓度升高下降，与对照组比较，差异有显著性（x^2=85．75-374．24，P〈0．01）。结论CGRP可以促进HaCaT细胞增殖，抑制其凋亡，且作用强度与浓度有关。
Objective To investigate the effect of calcitonin gene-related peptide （CGRP） on the proliferation and apopto sis of HaCaT cells. Methods Absorbance （A） of HaCaT cell treated with CGRP in different doses （range： 10^-7 - 10^-4 mmol/L） and cell growth curve was determined by MTT. Apoptosis rate was assessed with flow cytometry. Results Absorbance of H aCaT cell treated with CGRP increased significantly, compared with that of the control （F= 7.78, q = 3.53- 7.71, P〈0.05）. The apoptosis rate of HaCaT cells treated with CGRP was significantly lower than that of the control （x^2 =85.75-374.24,P〈 0.01）. Conclusion CGRP can promote HaCaT cell proliferation and inhibit apoptosis effectively. The effect is related to the concentration of CGRP. [
出处
《青岛大学医学院学报》
CAS
2008年第2期145-146,149,共3页
Acta Academiae Medicinae Qingdao Universitatis
