曼氏无针乌贼血细胞形态及分类

Classification and Morphological Observation on Haemocytes in Sepiella maindroni

采用不同固定剂和染色剂对曼氏无针乌贼的血细胞进行处理,利用组织学的手段对血细胞形态进行了观察、记数和测量.曼氏无针乌贼血细胞浓度平均为2.5×107cells/mL.根据细胞质中颗粒的多少、大小、折光性、染色特性及血细胞的大小、核质比等,将曼氏无针乌贼的血细胞分为二大类：透明细胞和颗粒细胞.透明细胞近圆形,直径7.65-8.28μm,细胞核卵圆形,较大,细胞质中颗粒很少,核质比约0.66,约占总细胞数的45.3%;颗粒细胞圆形,直径9.58-10.42μm,细胞质中有大量嗜酸性颗粒,核质比较小,约0.40,有些具双核,约占总细胞数的54.7%.研究了曼氏无针乌贼血细胞的形态和分类,为曼氏无针乌贼血细胞免疫的深入研究奠定了基础.

Study on the morphology of the haemocytes of the cuttlefish Sepiella maindroni was conducted by the method of histological staining in this paper.The average density of haemocytes was 2.5×107 cells/mL.The haemocytes of the cuttlefish S.maindroni were classified into two types,hyalinocytes and granulocytes,according to the characteristics of the haemocytes.Hyalinocytes with the size of 7.65~8.28 μm,were roundness,the nucleolus were also roundness surrounded by relatively small cytoplasm,there were little granule in cytoplast,the nucleus/cytoplasm（N/C） ratio was higher（0.66±0.10）,and the proportion of haemocytes was 45.3% in the total haemocytes.Granulocytes with the size of 9.58~10.42 μm were roundness,there were much granules in the endoplasm and the N /C ratio was lower（0.40±0.06）,some of the granulocytes had double nuclei,and the proportion of granulocytes was 54.7% in the total haemocytes.This research might be a foundation for further study on haemocytes immune of S.maindroni.