摘要
目的研究钙结合蛋白Calbindin-D28k在遗传性高钙尿结石（GHS）大鼠肾脏中的表达，并探讨其在特发性高钙尿症发病中的作用。方法分别取16只GHS大鼠与16只正常对照组（NC）大鼠新鲜肾脏组织，Western印迹检测Calbindin-D28k蛋白表达水平，实时荧光定量PCR检测其基因表达水平。结果GHS大鼠Calbindin-D28k蛋白表达水平显著高于NC大鼠（A值分别为0．49±0．02和0．20±0．01，P〈0．05），GHS组Calbindin-D28k mRNA表达水平是NC组的1．21倍[2^-（△△CT）值分别为1．21和1．0，P〉0．05]。结论Calbindin-D28k的表达上调可能是由GHS大鼠的维生素D受体高表达和高尿钙所引起，在尿钙重吸收中起重要作用，但不是导致特发性高钙尿症的关键分子。
Objective To study the expression level of calbindin-D28k, a kind of calcium binding protein, in the kidneys of genetic hypercalciuric stone-forming （GHS） rats and to investigate its role in idiopathic hypercalciuria （IH）. Methods Kidneys were taken out from 16 GHS rats and 6 normal control （NC） rats. Western blotting and real time quantitative PCR were used to detect the protein and mRNA expression levels of calbindin-D28k respectively. Results Western blotting showed that the A value of calbindin-D28k of the GHS rats was 0.49±0.02, significantly higher than that of the NC rats （0.20±0.01, P〈0.05）. The 2^-（△△CT） value of mRNA of calbindin-D＇28k of the GHS rats was 1.21, remarkably higher than that of the NC rats [with the of 2^-（△△CT） value of 1.0]. There was not significant difference in the ACT value between the two groups （ P 〉 0.05 ）. Conclusion The up-regulation of calbindin-D28k in the GHS rats is possibly caused by hyperexpression of VDR and hypercalcinuria, and plays an important role in urine calcium reabsorption; however, it is not the key protein that results in IH.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第20期1422-1424,共3页
National Medical Journal of China
基金
国家自然科学基金项目资助（30672100）
