高表达ΔNp73基因对大肠癌细胞生物学行为的影响及其机制研究

Impact of ΔNp73 Expression on Biological Activity of Human Colon Cancer

目的通过增强肿瘤细胞中外源性ΔNp73基因的表达,探讨该基因对大肠癌细胞生物学行为的影响。方法以人类大肠癌细胞株LOVO细胞为研究对象,将ΔNp73基因用脂质体法转染LOVO细胞,进行细胞生长曲线和流式细胞术分析,测定转染ΔNp73基因后的LOVO细胞的增殖能力与凋亡率;以Boyden小室体外侵袭实验检测ΔNp73基因转染前后LOVO细胞侵袭力变化,并用Western-blot法检测VEGF蛋白的表达。结果转染了ΔNp73基因后的LOVO细胞生长速度较未转染ΔNp73的LOVO细胞明显加快(P<0.01),凋亡率降低;Boyden小室体外侵袭实验显示LOVO-ΔNp73细胞穿膜数较空白对照组和LOVO-pcDNA3组增多,差异具有统计学意义(P<0.01)。与空白对照组相比,LOVO-ΔNp73细胞中VEGF表达较未转染ΔNp73基因的细胞明显增高(P<0.01)。结论ΔNp73过表达促进了大肠癌细胞的增殖和血管生成,增强了大肠癌的侵袭力。

Objective To investigate the relationship between the expression of △Np73 and the invasiveness of human colon cancer. Methods This study we used LOVO cell,a kind cell of human colon cacinoma cell, as the study objective. △Np73 was transferred into LOVO cell by liposome. The effect of △Np73 gene on cell growth was detected by trypan blue counter measurement, cell growth curve and flow cytometry, and invasive ability was assessed with Boyden chamber. Western-blot were employed to detect the expression of VEGF. Results The growth of LOVO-△Np73 cells were significantly increased, the invasive cell number was significantly larger in LOVO-△Np73 cells than in untransfected LOVO cells and LOVO-pcDNA3 o The expression of VEGF in the △Np73-LOVO was significantly higher than in untransfected LOVO cells（P〈0. 01 ）. Conclusion The overexpression of △Np73 promote the proliferation and vessel growth increase the ability of invasion of human colon cancer.