HSG和MMP-3在不同乳腺组织中的表达

Expression Characters of Hyperplasic Suppress Gene(HSG) and Matrix Metalloproteinase-3(MMP-3) in Different Breast Tissues

目的探讨新的增殖抑制基因(hyperplasia suppressor gene,HSG)和基质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)在不同乳腺组织中的表达。方法应用免疫组化方法检测唐山市工人医院58例乳腺癌标本,33例乳腺纤维瘤标本,30例乳腺增生标本和15例正常乳腺组织标本HSG和MMP-3的表达。结果58例乳腺癌组织中HSG和MMP-3的表达率分别为44.83%(26/58)和84.48%(49/58),与正常乳腺组织和良性乳腺纤维腺瘤比较,乳腺癌组HSG表达率下降,MMP-3表达率升高,差异有显著性(P<0.05);乳腺癌组HSG低表达,与淋巴结转移有关(P<0.05),与肿瘤大小、年龄和临床病理分级无关,MMP-3高表达与肿瘤大小和淋巴结转移等无关。另外,在乳腺癌组、乳腺增生组、乳腺纤维腺瘤组和正常乳腺组织中,HSG与MMP-3无明显相关性。结论HSG和MMP-3在不同乳腺组织中表达量的不同,提示HSG和MMP-3与乳腺癌的发生、发展有着密切关系。

Objective To research the expression of the novel hyperplasia suppressor gene （HSG） and matrix metalloproteinase-3（MMP-3） in different breast tissues. Methods Immunohistochemistry method was used to measure the expression of HSG and MMP-3. Fifty-eight cases were breast cancer samples, thirty-three cases were breast fibroadenoma samples, thirty cases were breast hyperplasia and fifteen samples of normal breast tissues. All these cases were collected up from the department of pathology of the Tangshan Worker＇s Hospital. Results The positive rate of HSG and MMP-3 was 44. 83% （26/58）and 84. 48%（49/58）in breast cancer tissues respectively. The positive expression rate of HSG was compared between normal breast group and breast fibroadenoma group, the expression rate of HSG decreased significantly, MMP-3 increased in the breast cancer group（P〈0. 05） ; the low level of HSG in breast cancer was correlated with lymph node metastasis（P〈0. 05）,was not correlated with the tumor size, age clinical pathological grade. The high level of MMP-3 was not associated with the tumor size and lymph node metastasis. On the other hand, there were not clear correlation between HSG and MMP-3 in breast cancer, breast hyperplasia, breast fibroadenoma and normal breast tissues. Conclusion The expression rate of HSG and MMP-3 was not equal in different breast tissues. It indicated that there was a close relationship between HSG, MMP-3 and the occurrence and development of breast cancer.