摘要
基因表达分析对于揭示基因功能、了解基因间相互关系、阐明植物生长发育过程中的生理生化调控机制起着至关重要的作用。综述了mRNA差异显示、cDNA-AFLP、基因芯片、EST数据分析、基因表达系列分析(SAGE)等全基因组基因表达分析技术的原理特点及在果树上的应用情况,并介绍了利用全基因组基因表达分析技术开展果树研究的思路。
Analysis of gene expression is very important to elucidate gene function, understand the interaction of genes and illustrate the mechanism of physiology and biochemistry in plant growth and development. This paper reviews the principles and characteristics of technology of genome-wide analysis of gene expression, including mRNA differential display, cDNA- AFLP, gene chip, expressed sequence tag （EST） database analysis, and serial analysis of gene expression （SAGE）. And the application of the technology in fruit trees is introduced. The ideas on how to study fruit trees with technology of genome-wide analysis of gene expression are suggested.
出处
《果树学报》
CAS
CSCD
北大核心
2008年第3期382-388,共7页
Journal of Fruit Science
基金
国家自然科学基金(30671432)
教育部新世纪优秀人才支持计划项目
关键词
果树
基因表达
基因芯片
表达序列标签
基因表达系列分析
Fruit trees
Gene expression
Gene chip
Expressed sequence tag
Serial analysis of gene expression
