一种植物表达载体的构建及其在金柑上的瞬时表达

Construction of a plant expression vector and transient gene expression in Kumquat (Fortunella crassifolia )

以植物表达载体pCambia1301的带内含子的GUS(iGUS)基因替换植物表达载体pBI121中的GUS基因,构建了以pBI121为基础载体并含有iGUS的植物表达载体pLZ13。农杆菌染色表明,pCamiba1301和pLZ13两载体中的iGUS基因均不会导致GUS染色反应。以金柑不同组织为材料,通过农杆菌介导开展瞬时表达研究,结果表明,pLZ13和pCambia1301两载体的iGUS在CaMV35S启动子调控下的表达没有差异,证明构建的pLZ13是一种同时适于瞬时表达和转基因研究的植物表达载体。

A plant expression vector, pLZ13, was constructed by replacing the GUS gene in pBI121 with the intron-GUS （iGUS） gene from pCambial301. GUS staining of Agrobacterium cells containing pCambial301 or pLZ13 indicated that GUS staining was prevented in both cases. A transient expression study was carried out with kumquat tissues as samples, and no difference was observed for the GUS expression patterns driven by the CaMV 35S promoters from the newly constructed vector pLZ13 and pCambial301. The vector pLZ13 was a plant expression vector, both suitable for transient gene expression and transgenic research.