摘要
目的研究隔药灸和电针对克罗恩病大鼠结肠IGF-I、IGF-IR、IGFBP-5蛋白及IGF-I mRNA的调节作用,探讨针灸治疗大鼠克罗恩病肠纤维化的作用机制。方法SD大鼠随机分为正常组、模型组、隔药灸组、电针组和药物组。采用三硝基苯磺酸制备大鼠克罗恩病模型,应用HE染色、Masson染色光镜下观察结肠病理组织学变化及胶原纤维增生情况;采用实时荧光定量聚合酶链反应检测结肠IGF-I mRNA的变化;采用免疫组织化学Envision法检测结肠IGF-I、IGF-IR、IGFBP-5蛋白表达变化。结果与正常组比较,模型组大鼠结肠组织胶原增生,IGF-I、IGF-IR、IGFBP-5蛋白表达均显著增加,IGF-I mRNA表达显著增加。经治疗后,与模型组比较隔药灸组、电针组、药物组大鼠结肠胶原增生均显著减少,隔药灸组较电针组作用显著。隔药灸组、电针组、药物组大鼠IGF-I、IGF-IR、IGFBP-5蛋白表达均显著降低;隔药灸组对IGF-I、IGFBP-5蛋白的下调作用优于电针组。隔药灸组、药物组IGF-I mRNA表达均显著降低,电针组变化不明显。结论隔药灸和电针能够下调结肠IGF-I、IGF-IR、IGFBP-5蛋白的异常表达,该作用可能是隔药灸和电针改善克罗恩病肠纤维化的重要机制。隔药灸能够下调IGF-I mRNA表达,对IGF-I、IGFBP-5蛋白的下调作用优于电针,该作用可能是其改善克罗恩病肠纤维化优于电针的机制之一。
Objective To explore the mechanism of acupuncture and moxibustion on treating fibrosis in colon of Crohn＇ s disease （CD） rats by investigating the regulating action of electro-acupuncture and herbs-partitioned moxibustion on the expressions of insulin- like growth factor-I （IGF-I） ,insulin-like growth factor-I receptor （IGF-IR） and insulin-like growth factor binding, protein-5 （IGFBP- 5 ）. Methods Sprague-Dawley rats were randomly divided into normal group, model group, herbs- partitioned moxibustion group, electro-acupuncture group and medication group. CD rat models were made by 2,4,6-trlnitrobenzene sulfonic acid. Pathologic histology changes of colon were observed under light microscope with HE stain and Masson stain. The expression of IGF-I mRNA was detected by real-time fluorescence quantitative polymerase chain reaction（FQ-PCR）. The expressions of IGF-I,IGF-IR and IGFBP-5 protein were determined by Envision immunohistochemical method. Results Compared with normal group, the collagen hyperplasy and expressions of IGF-I, IGF-IR, IGFBP-5 protein and IGF-I mRNA in colon were significantly increased in the model group. After treatment, collagen hyperplasy decreased significantly in herbs- partitioned moxibustion group, electro-acupuncture group and medication group compared with model group. The effect in herbs-partitioned moxibustion group is better than that in electro-acupuncture group. The expressions of IGF-I ,IGF-IR ,IGFBP-5 protein decreased significantly in herbs-partitioned moxibustion group, electro-acupuncture group and medication group, the expressions of IGF-I ,IGFBP-5 protein decreased more in herbs-partitioned moxibustion group than in electro-acupuncture group. The expression of IGF-I mRNA decreased significantly in herbs-partition moxibustion group and medication group while which in electro-acupuncture group had no statistical change. Conclusion Herbs-partitioned moxibustion and electro-acupuncture could decrease the abnormal protein expressions of IGF-I ,IGF-IR ,IGFBP-5, which may be the important mechanism of herbs-partition group and electro-acupuncture group in decreasing fibrosis of CD rats＇ colons. Herbs-partition moxibustion could decrease the expres- sions of IGF-I mRNA, and it is prior to the electro-acupuncture group in decreasing the expressions of IGF-I,IGFBP-5 protein which possibly lead to a better effect in improvement of fibrosis in herbs-partition moxibustion group.
出处
《上海针灸杂志》
2008年第5期37-40,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金资助项目(NO30400609)
上海市重点学科建设资助项目(T0302)
