甲基原薯蓣皂苷含量的高效液相色谱法测定

Determination of the Content of Methyl Protodioscin by HPLC

建立了测定甲基原薯蓣皂苷（MPD）含量的高效液相色谱法。以甲醇制样，采用氨基柱（200mm×4．6mm，5μm），以乙腈-水（体积比90：10）流动相等度洗脱，流速1.2mL·min^-1，柱温35℃，检测波长208nm；MPD在0．1008～1．008g·L^-1范围内线性关系良好（r=0．9998，n=6）；精密度实验的RSD为2．4％（n=6），重复性实验的RSD为1．6％（n=5），平均回收率为98．4％。3批MPD原料的批内平均含量均不少于92．89％（n=5），批间平均含量为93．78％（n=3）。

A HPLC method was established for assay of the content of methyl protodioscin （MPD） raw material. Methanol was used as solvent for sample preparation. Chromatographic separation was achieved on an amino group column（200 mm×4.6 mm, 5μm） by isocratic elution with acetonitrile- water（90 ： 10, by volume） as mobile phase at a flow rate of 1.2 mL/min with the column temperature at 35℃ and the detection wavelength at 208 nm. The linearity of MPD was between 0. 100 8 and 1. 008 g · L^-1. The RSD were 2.4% （ n = 6） in precision test and 1.6% （ n = 5） in reproducibility test and the average recovery was 98.4% . The intra-batch average content in 3 batches of MPD raw material was no less than 92.89% （n = 5）, and the inter-batch average content of MPD was 93.78% . The method could provide the foundation for quality control and new drug research of MPD.