IL-13基因多态性与RSV感染后婴幼儿喘息发病的关系

Association between IL-13 gene polymorphism and disease severity after respiratory syncytial virus infection

目的探讨IL-13Arg110Gln基因多态性与呼吸道合胞病毒(RSV)感染后喘息发作的关系。方法根据临床表现,将RSV感染者分为无喘息组和喘息组,取其颊黏膜细胞,提取DNA,用实时PCR法对IL-13Arg110Gln基因检测,分析基因多态性与喘息发生和病情之间的关系。结果在RSV感染患儿中存在IL-13Arg110Gln位点不同,在喘息组,A和G碱基的发生频率分别为58.92%、41.07%,而在无喘息组A和G碱基的发生频率则分别是44.39%、55.61%,两组比较差异有统计学意义。AA型较GG型患儿喘息发作病情重,住院天数多,差异有统计学意义。另外,AA型患儿IgE和嗜酸性细胞阳离子蛋白(ECP)水平明显高于GG型。结论IL-13Arg110Gln多态性AA基因型与RSV感染后婴幼儿喘息的发病相关。

Objectives To discuss the role of IL-13 gene polymorphism Arg110Gln in the pathogenesis of respiratory syncytial virus （RSV） infection. Methods RSV antibody was detected in 210 young children from the pars nasalis pharyngis by means of direct immunofluorescence essay. The patients were divided into two groups based on their clinical presentations： wheezing group （n = 112） and no-wheezing group （n = 98, including those without symptoms）. DNA was extracted from buccal mucosa cells, then real-time PCR is employed to analyze IL-13 gene. Results Genotype variants were found. In the wheezing group, Arg110 and Gln110 accounted for 58.92% and 41.07%, while in the no-wheezing group the proportion of Arg110 and Gln110 were 44.39% and 55.61%, respectively. There were significant statistical differences between the two groups （P 〈 0.01 ）. Severity of wheezing and duration of hospitalization were positively related to Arg110 of IL-13 gene polymorphism, moreover, level of IgE and eosinophil cationic protein in the children with Arg110 were higher than those with Gln110. Conclusions Arg110 of IL-13 gene polymorphism play a role in pathogenesis of infant wheezing after RSV infection.