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小麦杂交种与亲本之间穗下节间基因差异表达分析 被引量:5

Differential Gene Expression in Uppermost Internode between Wheat Hybrid and Its Parents
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摘要 以株高和穗下节间长度表现杂种优势的小麦杂交组合矮9×冀矮8号的杂种及其亲本为材料,应用cDNA-AFLP技术分析杂种、亲本的穗下节间基因差异表达情况。分离差异表达基因段后,对其克隆、测序并在GenBank进行Blast-x分析和功能推测,发现差异表达的基因包括分别受赤霉素和细胞分裂素诱导表达、与细胞分裂有关的基因cdc2和PAS1基因,它们在小麦杂种节间中分别特异表达和偏高亲表达。另有一个与细胞快速膨大有关的液泡质子泵H+-PPase基因在杂种中偏高亲表达。其他差异表达的基因包括蛋白激酶等与植物信号传递相关基因、与物质代谢相关的基因、参与基因转录调控的基因、与泛素参与的蛋白降解相关的基因等。小麦杂交种与亲本的穗下节间,大量基因涉及细胞分裂、膨大和物质代谢、转录调控等过程,初步推测它们的差异表达可能与穗下节间及株高杂种优势形成有关。 Heterosis is defined as the advantage of hybrid performance over its parents in growth and productivity. Previous studies showed that differential gene expression in hybrids and their parents is responsible for the heterosis. Plant hormones, especially GAs are found to be related to heterosis in development of shoot and plant height. However, information on systematic identification and characterization of the differentially expressed genes in shoots of hybrid and their parents are not found, and molecular evidence for the contribution of plant hormone to plant heterosis are also absent. The purpose of this paper was to investigate the differential gene express in stems of hybrid and their parents, and further, to investigate the possible role of genes participating hormone signaling in the formation of heterosis of plant stem. Using a wheat cross combination that exhibited vigorous heterosis in plant height, the differential gene expression in upper-most internode of wheat hybrids and their parents was analyzed by cDNA-AFLP. Ninety-eight differentially expressing cDNA fragments were obtained, which were sequenced and analyzed by Blast-x. Reverse Northern hybridization was performed in order to test the expression pattern of isolated ESTs in hybrid and their parents. The differentially expressed genes included a cdc2 gene and a PAS1 gene, which were induced by GAs and CTKs respectively and participated in plant cell division. The cdc2 gene was up-regulated in hybrid while the expression of PAS 1 in hybrid was near the higher parent. The differentially expressed genes also included kinase participating in signal transduodon, genes involved in metabolism and genes related to transcription regulations. It can be inferred that numerous genes were differentially expressed in the upper-most internode of hybrids and their parents, including genes participating in cell division and cell expansion, kinase related to signal transduction and so on. Reverse Northern blot analysis indicated that the expression pattern in our cDNA-AFLP analysis is credible. Since it has been much reported that genes participating in cell division and cell expansion are involved in plant stem growth and elongation, and genes that participate in gibberellins signals have been proved to be up-regulated in wheat hybrid and contributed to heterosis in wheat plant height, it can be modestly speculated that the differential expression of genes involved in phytohormone signals may be related to heterosis in wheat intenode elongation and plant height.
出处 《作物学报》 CAS CSCD 北大核心 2008年第5期770-776,共7页 Acta Agronomica Sinica
基金 国家自然科学基金项目(30671297) 国家基础研究发展计划(973计划)项目(2001CB1088) 教育部新世纪优秀人才支持计划(NCET-05-0131)
关键词 小麦 节间 CDNA-AFLP 基因差异表达 Wheat Internode cDNA-AFLP Differential gene expression
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参考文献32

  • 1Tasftaris S A. Molecular aspects of heterosis in plants. Plant Physiol, 1995, 94:362-370
  • 2孙其信.农作物杂种优势机理研究及展望[J].作物杂志,1998(4):31-31. 被引量:17
  • 3Sun Q X, Wu L M, Ni Z F, Meng F R, Wang Z K, Lin Z. Differential gene expression patterns in leaves between hybrids and their parental inbreds are correlated with heterosis in a wheat diallel cross. Plant Sci, 2004, 166:651-657
  • 4杨洪强,贾文锁,黄丛林,张大鹏.蛋白磷酸化参与湖北海棠根系中水分胁迫诱导的ABA积累[J].科学通报,2001,46(1):50-53. 被引量:26
  • 5Rood S B, Buzzeli R I, Mander L N, Pearce D, Pharis R P. Gibberellins: a phytohormonal basis for heterosis in maize. Science, 1988, 241:1216-1218
  • 6Bate N J, Rood S B, Blake T J. Gibberellins and heterosis in poplar. Can JBot, 1987, 66:1148-1152
  • 7吴晓俊,杜旻,翁颖琦,刘涤,胡之璧.UGPase of Astragalus membranaceus : cDNA Cloning, Analyzing and Expressing in Escherichia coli[J].Acta Botanica Sinica,2002,44(6):689-693. 被引量:9
  • 8Duca M. The spatial and temporal distribution of auxin and gibberellin in sunflower (Helianthus annuus L.). J Cell Mol Biol, 2006, 5:43-49
  • 9Bowen B, Mei G, Smith O, Carl R. Molecular profile for heterosis selection. USA, PCT/US2000/001422. 2000-01-19 [2007-06-28]. ttp://www.wipo.int/pctdb/en/wo.jsp ?wo=2000042838
  • 10Lu C M, Shen F S, Hu K. Interspecific heterosis in hybrids between Brassica napus and B. rapa Sabrai. J Genet Breed, 2000, 33:73-86

二级参考文献58

  • 1朱冰,黄大年,杨炜,薛锐,肖晗,田文忠,李良才,戴顺洪.利用基因枪法获得可遗传的抗除草剂转基因水稻植株[J].中国农业科学,1996,29(6):15-20. 被引量:90
  • 2黄大年 李敬阳 等.-[J].科学通报,1998,43:784-786.
  • 3WUX-J(吴晓俊) LiuD(刘涤) HuZ-B(胡之璧).UDP—glucose pyrophosphorylase[J].植物生理学通讯,2000,36:193-200.
  • 4Shan J-J(单俊杰),Wang S-C(王顺春),Liu D(刘涤),Hu Z-B(胡之璧).Progress ofchemical and pharmacological study of Astragalus polysaccharide.Acta Univ Trad Med SinPharmacol Shanghai(上海中医药大学学报),2000,14(3):61-65.(in Chinese with Englishabstract)
  • 5Du M(杜),Xiang D-J(向德军),Ding J-Y(丁家宜),Liu D(刘涤).Culture systemestablishment and chemical constituents analysis of hairy roots of Glycyrrhiza uralensisFisch.J Plant Resour Envir(植物资源与环境学报),2001,10(1):7-10.(in Chinese withEnglish abstract)
  • 6PengJ-S(彭佶松) ZhaoS-J(赵淑娟) WuX-J(吴晓俊) LiuD(刘涤) HuZ-B(胡之璧) XuZ-K(许政日岂).cDNA cloning and structural analysis of granule—bound starch synthasegene of hairy roots of Astragalus membranaceus[J].植物学报,2000,42:940-945.
  • 7Hu Z-B(胡之璧),Zheng Z-R(郑志仁),Li X-P(李幸平),Liu D(刘涤).Establishmentof hairy root cultures of Astragalus membranaceus and the extrinsic factors affectingtheir growth.Acta Bot Sin(植物学报),1998,40:448-452.(in Chinese with English abstract)
  • 8Lu S-D(卢圣栋).Advanced Experiment Protocol of Molecular Biology.Beijing:HigherEducation Press,1993.147-149.(in Chinese)
  • 9Elling L,Kula M R.Investigation of the UDP-glucose dehydrogenase reaction for acoupled assay of UDP-glucose pyrophosphorylase activities.Biotechnol AppalBiochem,1991,14:306-316.
  • 10Bradford M M.A rapid and sensitive method for the quantitation of microgramquantities of protein utilizing the principle of protein dye binding.AnalBiochem,1976,72:248-254.

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