秃杉优选单株的离体培养技术研究

In vitro culture and plant regeneration of Taiwania flousiana

在选育12年生秃杉优良种源的优良母株上,取其枝条的顶芽为外植体,进行离体培养技术研究。结果表明,以改良WPM＋BA 1.0 mg/L＋ZT 0.5 mg/L＋NAA 0.05 mg/L＋CA 50 mg/L＋S 3%为最适宜芽苗增殖培养基;改良WPM＋BA 0.5 mg/L＋ZT 0.5 mg/L＋NAA 0.1 mg/L＋CA 50 mg/L＋S 3%为最适宜芽苗壮苗生长培养基;最适宜生根培养基为1/2MS＋IBA 0.5 mg/L＋NAA 0.3 mg/L＋CA 100 mg/L＋S 2%;将生根的试管苗移栽于蛭石、珍珠岩、泥炭体积比为5∶3∶2的混合基质,控制温度为20-30℃,相对湿度85%-90%,保湿20-25 d,其间适当遮荫,35 d后成活率达86%以上。研究成果为秃杉优良种质保存利用提供了扩繁实用技术。

A study was conducted on in vitro culture and plant regeneration of Taiwania flousiana with the apical buds excised from the newly-grown shoots of the five-year-old elite maternal plant. Results showed that the modified WPM supplemented with 1.0 mg/L BA ＋ 0. 5 mg/L ZT ＋ 0.05 mg/L NAA ＋ 50 mg/L Citric acid ＋ 3% Sucrose was optimal to the tube-shoot multiplication, while the modified WPM with 0. 5 mg/L BA ＋ 0. 5 mg/L ZT ＋ 0. 1 mg/L NAA ＋ 50 mg/L Citric acid ＋3% Sucrose was suitable for the vigorous growth. 1/2MS with 0. 5 mg/L IBA ＋0. 3 mg/L NAA ＋ 100 mg/L Citric acid ＋ 2% Sucrose optimized the tube-shoot rooting induction. The plantlets were transplanted into a substrate containing with the vermiculate, perlite and peat, 5： 3：2 （v/v）. At 20 - 30℃ ,relative humidity of 85% - 90% for 20 - 25 days, the plantlets could grow well, and the mean of survival rate of the plantlets was up to 86% after 35 days.