慢性阻塞性肺疾病患者血清白介素-17、基质金属蛋白酶-9水平测定及其意义

Levels and Clinical Implicaton of IL-17,MMP-9 in Serum of Patients with Chronic Obstructive Pulmonary Diseases

目的:通过测定慢性阻塞性肺疾病患者及正常人血清中白介素-17(IL-17)、基质金属蛋白酶-9(MMP -9)水平,探讨血清IL-17、MMP-9含量变化及其在慢性阻塞型肺疾病发病中的意义。方法:对慢性阻塞型肺疾病急性加重期及缓解期患者各30例,健康对照组28例,分别采用酶联免疫吸附试验(ELISA)法测定三组人群血清IL- 17、MMP-9水平,并同时测第一秒用力呼气容积(FEV1)占预计值的百分比。结果:COPD急性加重期、缓解期患者血浆IL-17、MMP-9水平均高于健康对照组,差异有统计学意义(P<0.01);急性加重期血浆IL-17水平高于缓解期,差异有统计学意义(P<0.01);急性加重期血浆MMP-9水平高于缓解期,差异无统计学意义(P>0.05);COPD急性加重期、缓解期患者血浆IL-17与MMP-9水平无相关性P>0.05;急性加重期、缓解期患者血浆IL-17均与FEV1. 0%占正常预计值均呈负相关(r=-0.772,P<0.05;r=-0.49,P<0.05);急性加重期、缓解期患者血浆MMP-9水平与FEV1.0%占正常预计值均呈负相关(r=-0.669,P<0.05;r=-0.537,P<0.05)。结论:IL-17与MMP- 9参与了慢性阻塞性肺疾病的发生发展过程,IL-17、MMP-9的检测对于慢性阻塞性肺疾病患者诊断治疗有重要意义。

Objective： To investigate the changes of interleukin（IL） - 17 and matrix metalloproteinases - 9 （MMP - 9） in plasma of patients with chronic obstructive pulmonary disease （COPD） both during acute exacerbation and stable stage with and its relationship with respiratory function. Methods： The serum levels of IL- 17 and MMP- 9 were measured by ELISA in 30 cases of patients with COPD both during acute exacerbation and stable stage. Heathy nonsmoking volunteers were included as controls.The concentrations of IL-17, MMP- 9 in the sertum were measured by ELISA.Lung ventilatory function forced expiratory volume in one second （FEV1） were measured in all patients and healthy people. Results： The concentrations of IL- 17, MMP- 9 in patients with COPD during acute exacerbation were signifieantly higher than those in patients with COPD during stable stage and in the control Subjects（P〈 0.05）.The concentrations of IL -17, MMP - 9 in patients with COPD during stable stage were significandy higher than those in the control subjects （P〈0. 05） .The levels of IL-17 and MMP- 9 in patients with COPD both during acute exacerbation and stable stage correlated negatively with FEV1.0%pre. and it showed no correlative relationship between IL- 17 and MMP-9. Conclusion：The concentrations of IL-17, MMP-9 in patients of COPD were significantly increased. IL--1 7 , MMP -9 may play an important role in the airway flammation of COPD. It is very important for diagnosis and treatment of patients to measure serum IL- 17 ,MMP- 9 level.