摘要
通过室内培养试验,分别在不同时间取样分析,采用熏蒸-提取法测定土壤微生物生物量碳,提取液中的有机碳采用总有机碳分析仪测定,提取液中的氮采用凯氏消煮法测定;采用直接吸收法滴定测定土壤呼吸作用。研究了阿特拉津、甲磺隆、丁草胺3种除草剂对土壤微生物生物量碳、氮及呼吸强度的影响,揭示了3种除草剂污染的土壤中微生物生物量碳、氮的消长过程及土壤呼吸的动态变化。研究结果显示在较高使用浓度胁迫条件下,培养前20d3种除草剂都明显减少土壤微生物生物量碳、氮及抑制土壤呼吸。但这种抑制效应是随着时间而变化的,20d以后,它们对这些能表征土壤环境生态效应的生物学指标的影响随之降低。甲磺隆对微生物生物量碳、氮及呼吸作用等生物学指标的影响大于阿特拉津和丁草胺也可能与它们在土壤中的降解速率有关。
Laboratory incubation experiments were conducted to evaluated the affection of metsulfuron-methyl, butachlor and atrazine to microbial biomass carbon, biomass nitrogen and soil basal respiration in a paddy soil. The biomass C and N were determined by the chloroform fumigation and extraction method. The dissolved organic carbon content in the supernatant was measured on a Shimadzu TOC-500 automated Total Organic Carbon Analyzer. The Kjeldahl Nitrogen Determination method was applied to measure the dissolved organic nitrogen content in the supernatant. The direct absorption method was used to measure the soil basal respiration. Result showed that microbial biomass C, microbial biomass N and soil basal respiration rate decreased significantly in soil treated with three herbicides at a level of 10 mg·kg^-1 especially within the initial 20 days incubation, then this effect decreased after 20 days. The sequence of the affection of three herbicides on biological indicators for soil quality was: metsulfuron-methyl 〉 atrazine, butachlor. Maybe the degradation velocity of herbicides were one main reason conduced these phenomenon.
出处
《生态环境》
CSCD
北大核心
2008年第2期580-583,共4页
Ecology and Environmnet
基金
国家自然科学基金项目(40171051)
国家林业局森林生态环境重点实验室基金项目
关键词
除草剂
微生物生物量碳
微生物生物量氮
土壤呼吸
herbicides
microbial biomass carbon
microbial biomass nitrogen
soil basal respiration