肌母细胞体外培养技术的研究

Culture of myoblasts of skeletal muscle in vitro

目的探讨肌母细胞体外培养技术并鉴定其纯度。方法将肌肉组织消化、分离、纯化,接种到培养瓶中,3 d换液1次。用desmin抗体免疫组化染色鉴定细胞肌源性特性。结果每克肌肉组织可以培养0.85×107肌母细胞,97%肌母细胞desmin染色阳性。结论用本研究细胞培养技术培养肌母细胞纯度高,技术成熟,有广阔的应用前景。

Objective To investigate the efficiency of myoblast culture in vitro . Methods The specimens of skeletal muscle were divided equally into small ones and myoblasts were isolated by enzymatic digestion. The purity of the cells was determined by immunohistochemistry with a mouse anti-human desmin monoclonal antibody. Results The purity of myoblasts was 97 %. Conclusions Myoblasts with a high purity are cultured by the way of enzymatic digestion.