视网膜母细胞瘤相关抗凋亡因子的检测及其意义

Expression of anti-apoptosis factors in retinoblastoma tissue microarray

目的探讨凋亡相关因子Bag-1、p53和Bcl-2在视网膜母细胞瘤中的表达及意义。方法本研究用免疫组化SA法检测组织芯片中16例视网膜母细胞瘤和5例正常视网膜组织中Bag-1p、53和Bcl-2的表达。结果视网膜母细胞瘤组织芯片中Bag-l、p53和Bcl-12在肿瘤细胞胞浆均有高表达,在正常视网膜组织中表达阳性率低,差异显著(P<0.05)。结论Bag-l、P53和Bcl-2在视网膜母细胞瘤中均呈高表达,可能共同参与了视网膜母细胞瘤的发生。

Objective To investigate the expression and significance of Bag-1, p53, and Bcl-2 in tissue microarray. Methods 16 retinoblastoma and 5 normal retinal tisues were analyzed by immunohistochemical S-A system. Results Bag- 1,1353, and Bel-2 were over-expressed in cytoplasm in cancer tissue, while they were hardly seen in normal retinal cells. There was significant difference in positive rate of Bag-1, p53, and Bel-2 between the cancer tissues and the normal retinal tissues（ P 〈 0.05）. Condusions There are high expressions of Bag-1, p53, and Bel-2 in retinoblastoma, suggesting that Bag-1, p53 and Bcl-2 may be related to retinoblastoma.